Biovision Acetate Colorimetric Assay Kit

Description: The PARP1 Colorimetric Activity Assay Kit is designed to measure PARP1_x000D_activity for screening and profiling applications. PARP1 is known to catalyze the NAD-dependent_x000D_addition of poly(ADP-ribose) to histones. The key to the PARP1 Colorimetric Activity_x000D_Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1_x000D_reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated_x000D_substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate_x000D_is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to_x000D_produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.

Description: The PARP2 Colorimetric Activity Assay Kit is designed to measure PARP2_x000D_activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent_x000D_addition of poly(ADP-ribose) to histones. The key to the PARP2 Colorimetric Activity_x000D_Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP2_x000D_reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP2 biotinylated_x000D_substrate is incubated with an assay buffer that contains the PARP2 enzyme. Finally, the plate_x000D_is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to_x000D_produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.

Description: This product includes 1 ml of 10x Assay buffer, 0.2 ml of 10 x Reagent A, 0.2 ml of 10 x Reagent B and 0.5 ml of 10x Reagent C.