RNA sequencing (RNA-seq) is a well-validated tool for detecting gene fusions in fresh frozen tumors, whereas it is much more challenging to use it with formalin-fixed paraffin-embedded (FFPE) tumor samples. We evaluated the performance of RNA-seq to detect gene fusions in clinical FFPE tumor samples. Our assay identified all 15 spiked-in NTRK fusions from RNA reference material and 6 known fusions from 5 cancer cell lines. Limit of detection for the assay was assessed with a series of dilutions of RNA from the cell line H2228. These fusions can be detected when the dilution is down to 10%.
Good intra- and inter-assay reproducibility was observed in three specimens. For clinical validation, the assay detected 10 of 12 fusions initially identified by a DNA panel (covering 23 gene fusions) in clinical specimens (83.3% sensitivity), whereas one fusion (MET fusion) was identified in another 34 fusion-negative tumor specimens as determined by the DNA panel (negative prediction value of 94.3%). This MET fusion was confirmed by RT-PCR and Sanger sequencing, which demonstrated that this is a false negative for DNA panel. The assay also identified 26 extra fusions not covered by the DNA panel, and 20 of them (76.9%) were validated by RT-PCR and Sanger sequencing. Therefore, this RNA assay has reasonable performance and could be a complement to DNA-based next-generation sequencing assays.
Manufacture of rapid pressure-sensitive adhesive microwofit for pressure sensing and fluorescence detection specific to sequence bacterial plasmid gene sequences related to sepsis
Microbial resistance to antibiotics currently available poses a large threat in the global struggle against infections. An important step in determining the resistance to bacterial antibiotics can be a selective DNA sequence capture and fluorescence labeling. In this article, we demonstrate the manufacture of simple, robust and inexpensive microfluidic devices for DNA capture and fluorescence detection of a model antibiotic resistance genes sequence.
We, micromachine laser micromachine polymethacrylate polymethacrylate polymethacrylate polymethacrylate microchannels and closures using pressure-sensitive adhesive tapes. We then formed porous polymeric monoliths with DNA capture probes in these microchannels and used for capture, fluorescent labeling and fluorescence detection induced by the sequence of picomolar concentrations (PM) of genes targets of genes. Resistance to synthetic antibiotics and plasmids. The relative fluorescence for eluting peaks increased with a loaded target DNA concentration. We observed a higher fluorescence signal and percent recovery for synthetic target DNA with respect to plasmid DNA at the same loaded target concentration.
A non-target gene has been used for control experiments and product <3% capture relative to the same target concentration. The comprehensive analysis process includes the manufacture of devices has been completed in less than 90 minutes with a 30-hour detection limit. The simplicity of the manufacture of devices and a good capture capture of the DNA demonstrated here have an application potential with methods of extracting the bacterial plasmid DNA and counting to a single particles to facilitate Determining the susceptibility to antibiotics. Graphic summary.
The combined detection of immune genes to predict the prognosis of patients with non-small cell lung cancer
Lung cancer is one of the main causes of cancer-related death. In recent years, there has been growing interest in the fields of tumor and immunity. This study focused on the possible prognostic value of immune genes in patients with non-cell lung cancer. We used the Atlas of the Cancer Genome (TCGA) to download gene expression data and clinical information from lung adenocarcinoma (LUAD) and pulmonary pulmonary carcinoma (LUC). The list of immune genes has been downloaded from the Import database. We then built prognostic models of immune genes based on the regression analysis of the COX.
We also evaluated the clinical significance of the models via survival analysis, receiver operating characteristic curves (ROC) and an independent prognostic factor analysis. In addition, we analyzed the associations of prognostic models with mutation loads and neo-Engens. Using the OMNiBus (GEO) and Kaplan-Meier Genic Expression Databases, we evaluated the validity of the prognostic models. The PRONOSTIC model of LUAD included 13 immune genes and the prognostic model of LUSC contained 10 immune genes. High-risk patients based on prognostic models had a 5-year survival rate lower than low-risk patients.
Lymphocyte Activation Gene 3 Antibody |
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| DF14459-100ul | Affinity Biosciences | 100ul | 168 EUR |
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Description: IHC,IF/ICC,ELISA(peptide) |
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Lymphocyte Activation Gene 3 Antibody |
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| DF14459-200ul | Affinity Biosciences | 200ul | 210 EUR |
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Description: IHC,IF/ICC,ELISA(peptide) |
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Lymphocyte Activation Gene 3 Antibody |
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| MBS9631632-1mg | MyBiosource | 1mg | 375 EUR |
Lymphocyte Activation Gene 3 Antibody |
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| MBS9631632-5x1mg | MyBiosource | 5x1mg | 1545 EUR |
Lymphocyte Activation Gene 3 Antibody |
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| MBS9632130-1mg | MyBiosource | 1mg | 375 EUR |
Lymphocyte Activation Gene 3 Antibody |
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| MBS9632130-5x1mg | MyBiosource | 5x1mg | 1545 EUR |
Lymphocyte Activation Gene 3 Antibody |
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| MBS9629249-01mL | MyBiosource | 0.1mL | 260 EUR |
Lymphocyte Activation Gene 3 Antibody |
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| MBS9629249-02mL | MyBiosource | 0.2mL | 305 EUR |
Lymphocyte Activation Gene 3 Antibody |
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| MBS9629249-5x02mL | MyBiosource | 5x0.2mL | 1220 EUR |
Lymphocyte Activation Gene 3 Antibody |
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| MBS9629747-01mL | MyBiosource | 0.1mL | 260 EUR |
Lymphocyte Activation Gene 3 Antibody |
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| MBS9629747-02mL | MyBiosource | 0.2mL | 305 EUR |
Lymphocyte Activation Gene 3 Antibody |
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| MBS9629747-5x02mL | MyBiosource | 5x0.2mL | 1220 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx033099-400l | Abbexa | 400 µl | 518.75 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx033099-400ul | Abbexa | 400 ul | 627.6 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx033099-80l | Abbexa | 80 µl | 281.25 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx007220-100g | Abbexa | 100 µg | 275 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx007220 | Abbexa |
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx007220-10g | Abbexa | 10 µg | 175 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx007220-200g | Abbexa | 200 µg | 400 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx102894-100l | Abbexa | 100 µl | 262.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx102894 | Abbexa |
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx102894-1ml | Abbexa | 1 ml | 712.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx102894-200l | Abbexa | 200 µl | 325 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx102895-100l | Abbexa | 100 µl | 262.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx102895 | Abbexa |
|
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx102895-1ml | Abbexa | 1 ml | 725 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx102895-200l | Abbexa | 200 µl | 337.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx109319-100l | Abbexa | 100 µl | 162.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx109319 | Abbexa |
|
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx113548-100l | Abbexa | 100 µl | 612.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx113548 | Abbexa |
|
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx323649-100g | Abbexa | 100 µg | 250 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx323649 | Abbexa |
|
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx323649-50g | Abbexa | 50 µg | 187.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
|||
| abx130069-100l | Abbexa | 100 µl | 275 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx130069 | Abbexa |
|
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
|||
| abx130069-1ml | Abbexa | 1 ml | 775 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
|||
| abx130069-200l | Abbexa | 200 µl | 350 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
|||
| abx376382-96tests | Abbexa | 96 tests | 225 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx173393 | Abbexa |
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx173393-1ml | Abbexa | 1 ml | 275 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx211362-100l | Abbexa | 100 µl | 350 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| 20-abx211362 | Abbexa |
|
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Lymphocyte Activation Gene 3 (LAG3) Antibody |
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| abx211362-50l | Abbexa | 50 µl | 250 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody (PE) |
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| abx414103-200l | Abbexa | 200 µl | 487.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody (RPE) |
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| abx414103-100tests | Abbexa | 100 tests | 710.4 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody (RPE) |
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| abx414104-25tests | Abbexa | 25 tests | 376.8 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody (HRP) |
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| abx108468-100g | Abbexa | 100 µg | 362.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody (HRP) |
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| 20-abx108468 | Abbexa |
|
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Lymphocyte Activation Gene 3 (LAG3) Antibody (HRP) |
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| abx108468-20g | Abbexa | 20 µg | 162.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody (HRP) |
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| abx108468-50g | Abbexa | 50 µg | 250 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody (FITC) |
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| abx107047-100g | Abbexa | 100 µg | 362.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody (FITC) |
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| 20-abx107047 | Abbexa |
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Lymphocyte Activation Gene 3 (LAG3) Antibody (FITC) |
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| abx107047-20g | Abbexa | 20 µg | 162.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody (FITC) |
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| abx107047-50g | Abbexa | 50 µg | 250 EUR |
Lymphocyte Activation Gene 3 Antibody HRP Conjugated |
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| MBS9461123-01mL | MyBiosource | 0.1mL | 595 EUR |
Lymphocyte Activation Gene 3 Antibody HRP Conjugated |
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| MBS9461123-5x01mL | MyBiosource | 5x0.1mL | 2525 EUR |
Lymphocyte Activation Gene 3 Antibody Cy3 Conjugated |
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| MBS9461125-01mL | MyBiosource | 0.1mL | 595 EUR |
Lymphocyte Activation Gene 3 Antibody Cy3 Conjugated |
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| MBS9461125-5x01mL | MyBiosource | 5x0.1mL | 2525 EUR |
Lymphocyte Activation Gene 3 Antibody FITC Conjugated |
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| MBS9461124-01mL | MyBiosource | 0.1mL | 595 EUR |
Lymphocyte Activation Gene 3 Antibody FITC Conjugated |
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| MBS9461124-5x01mL | MyBiosource | 5x0.1mL | 2525 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody (Biotin) |
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| abx105630-100g | Abbexa | 100 µg | 362.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody (Biotin) |
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| 20-abx105630 | Abbexa |
|
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Lymphocyte Activation Gene 3 (LAG3) Antibody (Biotin) |
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| abx105630-20g | Abbexa | 20 µg | 162.5 EUR |
Lymphocyte Activation Gene 3 (LAG3) Antibody (Biotin) |
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| abx105630-50g | Abbexa | 50 µg | 250 EUR |
Lymphocyte activation gene 3 protein (LAG3) Antibody |
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| abx341054-100l | Abbexa | 100 µl | 400 EUR |
Lymphocyte activation gene 3 protein (LAG3) Antibody |
|||
| abx341054-50l | Abbexa | 50 µl | 287.5 EUR |
Lymphocyte Activation Gene 3 Protein (LAG3) Antibody |
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| abx433580-100g | Abbexa | 100 µg | 387.5 EUR |
Lymphocyte Activation Gene 3 Protein (LAG3) Antibody |
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| abx234676-100g | Abbexa | 100 µg | 350 EUR |
Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody |
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| CAU25527-100ul | Biomatik Corporation | 100ul | 241.9 EUR |
Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody |
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| CAU25527-200ul | Biomatik Corporation | 200ul | 302.4 EUR |
Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody |
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| CAU25528-100ul | Biomatik Corporation | 100ul | 229.4 EUR |
Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody |
|||
| CAU25528-200ul | Biomatik Corporation | 200ul | 287.3 EUR |
Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody |
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| CAU25529-100ul | Biomatik Corporation | 100ul | 222.7 EUR |
Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody |
|||
| CAU25529-200ul | Biomatik Corporation | 200ul | 278.9 EUR |
Rabbit anti-human Lymphocyte activation gene 3 polyclonal Antibody |
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| MBS7004076-005mg | MyBiosource | 0.05mg | 190 EUR |
Rabbit anti-human Lymphocyte activation gene 3 polyclonal Antibody |
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| MBS7004076-01mg | MyBiosource | 0.1mg | 270 EUR |
Rabbit anti-human Lymphocyte activation gene 3 polyclonal Antibody |
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| MBS7004076-5x01mg | MyBiosource | 5x0.1mg | 1205 EUR |
Monoclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MAB663Hu21 | Cloud-Clone | 100ul | 246 EUR |
Monoclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MAB663Hu22 | Cloud-Clone | 100ul | 253 EUR |
Monoclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2138968-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2006574-01mL | MyBiosource | 0.1mL | 170 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2006574-02mL | MyBiosource | 0.2mL | 215 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2006574-05mL | MyBiosource | 0.5mL | 350 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2006574-1mL | MyBiosource | 1mL | 425 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2006574-5mL | MyBiosource | 5mL | 1160 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2004765-01mL | MyBiosource | 0.1mL | 170 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2004765-02mL | MyBiosource | 0.2mL | 205 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2004765-05mL | MyBiosource | 0.5mL | 335 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2004765-1mL | MyBiosource | 1mL | 415 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2004765-5mL | MyBiosource | 5mL | 1130 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2027431-01mL | MyBiosource | 0.1mL | 175 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2027431-02mL | MyBiosource | 0.2mL | 220 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2027431-05mL | MyBiosource | 0.5mL | 365 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2027431-1mL | MyBiosource | 1mL | 445 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| MBS2027431-5mL | MyBiosource | 5mL | 1220 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| PAB663Hu01 | Cloud-Clone | 100ul | 232 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| PAB663Mu01 | Cloud-Clone | 100ul | 239 EUR |
Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| PAB663Ra01 | Cloud-Clone | 100ul | 252 EUR |
Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody (Rat) |
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| 4-PAB663Ra01 | Cloud-Clone |
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|
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Description: A Rabbit polyclonal antibody against Rat Lymphocyte Activation Gene 3 (LAG3) |
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Recombinant Antibody to Lymphocyte Activation Gene 3 (LAG3) |
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| RAB663Hu21 | Cloud-Clone | 100ul | 399 EUR |
Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody (Human) |
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| 4-PAB663Hu01 | Cloud-Clone |
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|
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Description: A Rabbit polyclonal antibody against Human Lymphocyte Activation Gene 3 (LAG3) |
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Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody (Mouse) |
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| 4-PAB663Mu01 | Cloud-Clone |
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|
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Description: A Rabbit polyclonal antibody against Mouse Lymphocyte Activation Gene 3 (LAG3) |
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Lymphocyte Activation Gene 3 (4Z17) Rabbit Monoclonal Antibody |
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| E28M3484 | EnoGene | 100ul | 295 EUR |
Lymphocyte activation gene 3 protein |
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| AP84383 | SAB | 1mg | 2640 EUR |
Lymphocyte activation gene 3 protein |
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| AP84823 | SAB | 1mg | 2640 EUR |
Lymphocyte activation gene 3 protein |
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| AP85011 | SAB | 1mg | 2640 EUR |
Lymphocyte activation gene 3 protein |
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| MBS7043006-001mg | MyBiosource | 0.01mg | 1285 EUR |
Lymphocyte activation gene 3 protein |
|||
| MBS7043006-005mg | MyBiosource | 0.05mg | 1845 EUR |
Lymphocyte activation gene 3 protein |
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| MBS7043006-01mg | MyBiosource | 0.1mg | 2780 EUR |
Lymphocyte Activation Gene 3 (11N12) Rabbit Monoclonal Antibody |
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| E28M3256 | EnoGene | 100ul | 295 EUR |
Rabbit anti-human Lymphocyte activation gene 3 polyclonal Antibody, FITC |
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| MBS1491858-005mg | MyBiosource | 0.05mg | 190 EUR |
Rabbit anti-human Lymphocyte activation gene 3 polyclonal Antibody, FITC |
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| MBS1491858-01mg | MyBiosource | 0.1mg | 270 EUR |
Rabbit anti-human Lymphocyte activation gene 3 polyclonal Antibody, FITC |
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| MBS1491858-5x01mg | MyBiosource | 5x0.1mg | 1205 EUR |
Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody (Rat), PE |
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| 4-PAB663Ra01-PE | Cloud-Clone |
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|
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Description: A Rabbit polyclonal antibody against Rat Lymphocyte Activation Gene 3 (LAG3). This antibody is labeled with PE. |
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Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody (Rat), APC |
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| 4-PAB663Ra01-APC | Cloud-Clone |
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|
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Description: A Rabbit polyclonal antibody against Rat Lymphocyte Activation Gene 3 (LAG3). This antibody is labeled with APC. |
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Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody (Rat), Cy3 |
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| 4-PAB663Ra01-Cy3 | Cloud-Clone |
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|
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Description: A Rabbit polyclonal antibody against Rat Lymphocyte Activation Gene 3 (LAG3). This antibody is labeled with Cy3. |
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Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody (Rat), HRP |
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| 4-PAB663Ra01-HRP | Cloud-Clone |
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|
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Description: A Rabbit polyclonal antibody against Rat Lymphocyte Activation Gene 3 (LAG3). This antibody is labeled with HRP. |
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Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody (Human), PE |
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| 4-PAB663Hu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Lymphocyte Activation Gene 3 (LAG3). This antibody is labeled with PE. |
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Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody (Mouse), PE |
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| 4-PAB663Mu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Lymphocyte Activation Gene 3 (LAG3). This antibody is labeled with PE. |
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Lymphocyte Activation Gene 3 (LAG3) Polyclonal Antibody (Rat), FITC |
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| 4-PAB663Ra01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Lymphocyte Activation Gene 3 (LAG3). This antibody is labeled with FITC. |
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The analysis of the ROC curve has demonstrated the accuracy of the prediction of the prognostic models, because the area under the curve (AUC) was 0.742, 0.707 and 0.711 for LUAD, and 0.6668, 0.703 and 0.668 for LUSC, when the planned survival time were 1, 3 and 5 years, respectively. Mutation load analysis showed that the mutation level was associated with the risk score in LUAD patients. The analysis based on the geo and kaplan-meier tracer has demonstrated the prognostic validity of the models. As a result, LUAD and LUSC immune gene models can predict the prognosis. A complementary study of these genes can allow us to better distinguish between LUAD and LUSC and lead to an improvement in immunotherapy for lung cancer.