Diagnostic Performances of Different Genome Amplification Assays for the Detection of Swine Vesicular Disease Virus in Relation to Genomic Lineages That Circulated in Italy

Over the last 25 years, a swine vesicular disease (SVD) has occurred in Italy mainly subclinic. Therefore, regular tests of faecal samples from suspected assets and high rolling premises were fundamental to identifying the circulation of the virus and performing SVD eradication. In this study, we evaluated the diagnostic performance of six genomic amplification methods, using positive fecal samples of 78 different epidemics (1997-2014), which included different lines. Comparison of three RT-PCRS, designed to amplify the same part of 154 NT of the 3D gene, demonstrated that a conventional and real-time test based on the Sybr green detection test showed the highest diagnostic sensitivity, detecting All samples, while real-time Taqman-based test failed three cases due to two incompatibles in the target sequence of the probe.

The specificities of diagnosis and analysis were optimal because 300 negative field samples and other enteroviruses reacted negative. Three other tests evaluated, previously described, were an inverse isothermal amplification on the 3D reverse transcriptase (Lamp RT) and two real-time RT-PCR targeting on the region of 5’UTR. Here, the presence of multiple imbalances in the probe and the primers reduced the diagnostic performance and two of the analyzes could not detect viruses of a sub-lineage. These results emphasize that the choice of tests using fewer nucleotide objectives has contributed considerably to the success of the SVD eradication plan. Acanthamoeba is a living amoeba of extended genetic diversity. This can cause infectious keratitis (IK), which can also be caused by bacteria, fungi and viruses. A high diagnostic sensitivity is essential for establishing early diagnosis of the keratitis associated with Acanthamoeba. Here we have studied the applicability of new generation sequencing (NGS) – detection of ribosomal genes and the differentiation of ribosomal genes (16s-18) (16s-18) with respect to a specific real-time PCR for detection of 'Acanthamoeba Two hundred dna corneal rappings extracts and projected by Acanthamoeba-specific. Real-time PCR has been analyzed using an internal NGS dosage.

The joint regression model (JRM) is used to describe the changes in the trend in many applications and relies on the detection of participation points (changePoints). However, the methods of detecting existing join points, namely the search methods of the grid (GS) – are demanding in a manner comprising and, consequently, the maximum number of calculable join points is limited. In this document, we have developed a sealing model of the genetic algorithm (GAJP) in which an explicitly decoupled computer procedure for optimization and regression is used to integrate a binary genetic algorithm into the JRM for optimal detection point of participation. The combinations of participation points were represented in the form of binary chromosomes and genetic operations were carried out to determine the optimal solution by minimizing the Fitness function, the Bayesian information criterion (BIC) and BIC3.

New genome sequence detection via natural vector convex hull method

It remains difficult to find existing but undiscovered genome sequence mutations or predicting the potential mutations of genome sequence based on actual sequence data. Motivated by this, we develop approaches to detect new non-discovered genome sequences. Because the discovery of new genome sequences through biological experiments is with high resource intensity, we want to achieve the new genome sequence detection task mathematically.

However, little literature tells us how to detect new non-discovered genome sequence mutations mathematically. We form a new frame based on a natural vector convex hull method that performs an alignment-free sequence analysis. Our newly developed approaches, random algorithm permutation with penalty (rap) and random algorithm permutation with sanction and costrising search (RAPCOS), use the properties of geometry captured by natural vectors. In our experience, we discover a genome sequence of mathematically human immunodeficiency of human immunodeficiency using certain real HIV genome sequences. Significantly, the proposed methods are applicable to the resolution of the new genome sequence detection challenge and many good properties, such as robustness, fast convergence and rapid calculation.

In situ detection of adeno-associated viral vector genomes with fish-fish

Gene therapy with recombinant inviolized virus vectors (AAV) is a promising modality for the treatment of various human diseases. Nevertheless, there are still important gaps in our understanding of the biology of AAV vectors, because in part to the lack of robust methods to follow the Capsides and genomes AAV. In this study, we describe a new application of the amplification of the fluorescence exchange of the in situ hybridization fluorescence exchange (saber-fish) which allowed the visualization and quantification of individual AAV genomes after the administration of Vector in the mouse.

Single Firefly Luciferase Reporter Gene Assay Kit
K2236-100	ApexBio	100T	68 EUR
Description: Transcriptional Regulation|Reporter Gene
Single Firefly Luciferase Reporter Gene Assay Kit
K2236-1000	ApexBio	1000T	336 EUR
Description: Transcriptional Regulation|Reporter Gene
Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
12518-1plate	AAT Bioquest	1 plate	166 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
12519-10plates	AAT Bioquest	10 plates	446 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
12520-100plates	AAT Bioquest	100 plates	3321 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
Amplite® Luciferase Reporter Gene Assay Kit *Maximized Luminescence*
12519	AAT Bioquest	10 plates	455 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12530-1plate	AAT Bioquest	1 plate	222 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12531-10Plates	AAT Bioquest	10 Plates	846 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*
12532-100plates	AAT Bioquest	100 plates	4447 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12535-1plate	AAT Bioquest	1 plate	222 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12536-10plates	AAT Bioquest	10 plates	1070 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*
12537-100plates	AAT Bioquest	100 plates	4447 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
Luciferase Reporter Assay Kit
55R-1540	Fitzgerald	200 assays	180 EUR
Description: Assay Kit for detection of Luciferase Reporter in the research laboratory
Luciferase Reporter Assay Kit
K2181-200	ApexBio	200 assays	130 EUR
Luciferase Reporter Assay Kit
K801-200	Biovision	each	235.2 EUR
Luciferase Reporter Assay Kit
GWB-AXR371	GenWay Biotech	200 assays	Ask for price
Amplite® Renilla Luciferase Reporter Gene Assay Kit *Maximized Luminescence*
12536	AAT Bioquest	10 plates	1092 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
Dual Luciferase Reporter Assay Kit
DL101-01	Vazyme	100 rxn	90.5 EUR
Dual Luciferase Reporter Assay Kit
DL101-01-100rxns	Vazyme	100 rxns	93.71 EUR
SRE Luciferase Reporter Lentivirus
78627	BPS Bioscience	500 µl x 2	835 EUR
Description: The SRE (Serum Response Element) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the Serum Response Element located upstream of the minimal TATA promoter . After transduction, activation of the MAPK/ERK signaling pathway in the target cells can be monitored by measuring the luciferase activity.
Myc Luciferase Reporter Lentivirus
78628	BPS Bioscience	500 µl x 2	835 EUR
Description: The Myc Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the Myc response element located upstream of the minimal TATA promoter and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the Myc signaling pathway in the target cells can be monitored by measuring the luciferase activity.
p53 Luciferase Reporter Lentivirus
78666	BPS Bioscience	500 µl x 2	835 EUR
Description: The p53 Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by p53 response elements located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, p53-regulated gene expression in the target cells can be monitored by measuring the luciferase activity.
HRE Luciferase Reporter Lentivirus
78668	BPS Bioscience	500 µl x 2	835 EUR
Description: The Hypoxia Response Element (HRE) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by four copies of a hypoxia response elements (HRE) located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the induction of hypoxia in the target cells can be monitored by measuring the luciferase activity.
ARE Luciferase Reporter Lentivirus
79869	BPS Bioscience	500 µl x 2	875 EUR
Description: The Nrf2 antioxidant response pathway plays an important role in the cellular antioxidant defense. Nrf2, a basic leucine zipper transcription factor, induces the expression of antioxidant and phase II enzymes by binding to the ARE (antioxidant response element) region of the gene promoter. Under basal conditions, Nrf2 is retained in the cytosol by binding to the cytoskeletal protein Keap1. Upon exposure to oxidative stress or other ARE activators, Nrf2 is released from Keap1 and translocates to the nucleus, where it can bind to the ARE, leading to the expression of antioxidant and phase II enzymes that protect the cell from oxidative damage. The ARE Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by ARE located upstream of the minimal TATA promoter. After transduction, activation of the Nrf2 antioxidant response pathway in the target cells can be monitored by measuring the luciferase activity.
TEAD Luciferase Reporter Lentivirus
79833	BPS Bioscience	500 µl x 2	875 EUR
Description: The Hippo pathway regulates cell proliferation and cell death. It is activated by high cell density and cell stress to stop cell proliferation and induce apoptosis. The mammalian Hippo pathway comprises MST kinases and LATS kinases. When the Hippo pathway is activated, MST kinases phosphorylate LATS kinases, which phosphorylate transcriptional co-activators YAP and TAZ. Unphosphorylated YAP and TAZ remain in nucleus and interact with TEAD/TEF transcriptional factors to turn on cell cycle-promoting gene transcription. However, when phosphorylated, YAP and TAZ are recruited from the nucleus to the cytosol, so that the YAP and TAZ-dependent gene transcription is turned off. Dysfunction of the Hippo pathway is frequently detected in human cancer and its down-regulation correlates with the aggressive properties of cancer cells and poor prognosis. The TEAD Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the TEAD response elements located upstream of the minimal TATA promoter. After transduction, activation of the Hippo pathway in the target cells can be monitored by measuring the luciferase activity._x000D_
STAT3 Luciferase Reporter Lentivirus
79744	BPS Bioscience	500 µl x 2	860 EUR
Description: The STAT3 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of STAT3-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the STAT3 signaling pathway in the target cells can be monitored by measuring the luciferase activity._x000D_
STAT5 Luciferase Reporter Lentivirus
79745	BPS Bioscience	500 µl x 2	835 EUR
Description: The STAT5 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of STAT5-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the STAT5 signaling pathway in the target cells can be monitored by measuring the luciferase activity.
NF-κB Luciferase Reporter Lentivirus
79564	BPS Bioscience	500 µl x 2	875 EUR
Description: The NF-κB Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After transduction, activation of the NF-κB signaling pathway in the target cells can be monitored by measuring the luciferase activity.
CRE/CREB Luciferase Reporter Lentivirus
79580	BPS Bioscience	500 µl x 2	835 EUR
Description: The main role of the cAMP response element, or CRE, is mediating the effects of Protein Kinase A (PKA) by way of transcription. Upon phosphorylation, CREB forms a functionally active dimer that binds the CRE element within the promoters of target genes and activates transcription. CRE is at the focus of many extracellular and intracellular signaling pathways, including cAMP, calcium, GPCR (G-protein coupled receptors) and neurotrophins. The cAMP/PKA signaling pathway is critical to numerous life processes in living organisms.The CRE/CREB Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized cAMP response element (CRE) located upstream of the minimal TATA promoter. After transduction, activation of the cAMP/PKA signaling pathway in the target cells can be monitored by measuring the luciferase activity.
Bald VSV Delta G (Luciferase Reporter)
78636-1	BPS Bioscience	100 µl	395 EUR
Description: The bald VSV Delta G (Luciferase Reporter) was produced without envelope glycoproteins. It contains the firefly luciferase gene as the reporter. The bald VSV Delta G (Luciferase Reporter) can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.
Bald VSV Delta G (Luciferase Reporter)
78636-2	BPS Bioscience	500 µl x 2	1995 EUR
Description: The bald VSV Delta G (Luciferase Reporter) was produced without envelope glycoproteins. It contains the firefly luciferase gene as the reporter. The bald VSV Delta G (Luciferase Reporter) can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.
HRE Luciferase Reporter-HeLa Cell Line
RC1018	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The HRE Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the hypoxia response element (HRE). In response to hypoxia (low oxygen), HREs of target genes are recognized and regulated by the hypoxia-inducible factors (HIFs) which belong to the family of basic helix-loop-helix transcription factors and form heterodimeric complex comprising the alpha subunit (HIF-1 alpha, HIF-2 alpha and HIF-3 alpha) and beta subunit (Arnt1, Arnt2 and Arnt3), among which HIF-1 alpha and HIF-2 alpha are predominant isoforms. Activation of HIFs can also be mediated by chemical hydroxylase inhibitors as hypoxia mimetics including the iron chelator desferrioxamine and cobalt chloride.The HRE induction by cobalt chloride is shown in Figure 1.
p53 Luciferase Reporter-HeLa Cell Line
RC1026	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The p53 Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the p53 response element. p53 is a tumor suppressor that plays a crucial role in apoptosis and anticancer mechanisms. p53 reporter system is designed to monitor the p53-mediated signaling pathways.The p53 induction by doxorubicin is shown in Figure 1.
MRE Luciferase Reporter HEK293 Cell Line
RC1037	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The MRE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the metal response element (MRE). MRE is targeted by MRE-binding transcription factor-1 (MTF-1) which is a zinc finger transcription factor and plays a major role in induction of metallothionein gene expression in response to cellular stress caused by heavy metals such as zinc and cadmium. The MRE induction by ZnSO4 is shown in Figure 1.
Nrf2 Luciferase Reporter-MCF7 Cell Line
RC1017	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The Nrf2 Luciferase Reporter cell line is a stably transfected MCF7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the antioxidant response element (ARE). ARE is known to regulate expression and induction of various detoxifying enzyme genes in response to antioxidants and xenobiotics, and is primarily regulated by the Keap1-Nrf2 pathway in which induction and nuclear translocation of Nrf2 mediated by antioxidants and xenobiotics results in the binding of Nrf2 to ARE leading to the expression of defensive genes. The Nrf2 induction by dimethyl fumarate (DMF) is shown in Figure 1.
ATF6 Luciferase Reporter-HeLa Cell Line
RC1038	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The ATF6 Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the activating transcription factor 6 (ATF6)-response element. ATF6 is a member of the basic-leucine zipper transcription factor family, which is located in the endoplasmic reticulum (ER) membranes and plays a central role in transcriptional activation of ER molecules.  The ATF6 induction by Tunicamycin is shown in Figure 1.
STAT1 Luciferase Reporter-HeLa Cell Line
RC1012	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	4536 EUR
Description: The STAT1 Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the interferon (IFN) gamma activation sequence-based STAT1 response element, so that the cell line is designed to measure the transcriptional activity of STAT1. As a transcription factor, Signal Transducer and Activator of Transcription 1 (STAT1) is activated through phosphorylation at tyrosine 701 in response to various cytokines and growth factors such as IFN-alpha, IFN-gamma, IL-6, EGF and PDGF. The phosphorylated STAT1 forms homodimers or heterodimers with STAT3, and the dimers translocate to nucleus in which DNA binding/promoter induction occurs. The STAT1 induction by IFN-gamma is shown in Figure 1.
SRE Luciferase Reporter-HEK293 Cell Line
RC1032	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	2772 EUR
Description: The SRE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the serum response element (SRE). The SRE reporter cell line is designed to monitor MAPK/ERK activity and can be used for studying GPCR-linked MAPK/ERK signaling pathways as well as screening of agonists, antagonists or signaling inhibitors related with the MAPK/ERK signaling pathways. Functional activity of the cell line has been validated by serum stimulation assay (Figure 1).
CRE Luciferase Reporter-HEK293 Cell Line
RC1034	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	2772 EUR
Description: The CRE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the cAMP response element (CRE). The CRE   cell line is designed to monitor the cAMP/PKA signaling pathways and can be used for studying GPCR-linked cAMP/PKA signaling pathways as well as screening of agonists, antagonists or signaling inhibitors related with the cAMP/PKA signaling pathways. Functional activity of the cell line has been validated by serum stimulation assay (Figure 1).
SBE Luciferase Reporter-HEK293 Cell Line
RC1036	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The SBE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the smad binding element (SBE). SMADs are intracellular signaling mediators that transduce extracellular signals from transforming growth factor beta (TGF-beta) ligands to the nucleus where they activate downstream gene transcription. The TGF-beta signaling pathway is involved in many cellular processes in both the adult organism and the developing embryo including cell growth, cell differentiation, apoptosis, cellular homeostasis and other cellular functions. The SBE induction by TGF-beta is shown in Figure 1.
NFAT Luciferase Reporter-HEK293 Cell Line
RC1010	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The NFAT Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Nuclear Factor of Activated T-cells (NFAT) response element, so that the cell line is designed to measure the transcriptional activity of NFAT. NFAT is a transcription factor originally found in activated T lymphocytes, and is now known to regulate not only T cell activation and differentiation but also the function of other immune cells including dendritic cells, B cells and megakaryocytes. The NFAT induction by calcium ionophore A23187 is shown in Figure 1.
NFAT Luciferase Reporter-RAW264.7 Cell Line
RC1011	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The NFAT Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Nuclear Factor of Activated T-cells (NFAT) response element, so that the cell line is designed to measure the transcriptional activity of NFAT. NFAT is a transcription factor originally found in activated T lymphocytes, and is now known to regulate not only T cell activation and differentiation but also the function of other immune cells including dendritic cells, B cells and megakaryocytes. The NFAT induction by calcium ionophore A23187 is shown in Figure 1.
iNOS Luciferase Reporter-RAW264.7 Cell Line
RC1015	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The iNOS Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the iNOS promoter. Inducible nitric oxide synthase (iNOS) is an inducible enzyme that catalyzes the production of nitric oxide (NO) from L-arginine. NO is one of the smallest signaling molecules that can diffuse into the cell and is involved in various physiological functions, pathogenesis of septic shock, many diseases associated with autoimmunity, and  tumorigenesis. iNOS gene is generally known to be induced by various proinflammatory cytokines and pathogen-associated molecular patterns such as Toll-like receptor (TLR) ligands. The iNOS induction by lipopolysaccharide (LPS), the TLR4 ligand, is shown in Figure 1.
ISRE Luciferase Reporter-RAW264.7 Cell Line
RC1041	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The ISRE Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Interferon-Stimulated Response Element (ISRE), so that the cell line is designed to monitor the JAK/STAT signaling pathway activity. This cell line can be activated by type I IFNs as well as certain Toll like receptor ligands capable of induction of IRFs such as TLR3 ligand-poly (I:C). Functional activity of the cell line has been validated by poly (I:C) (Figure 1).
ISRE Luciferase Reporter-HEK293 Cell Line
RC1043	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The ISRE Luciferase Reporter cell line is a stably transfected HEK293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Interferon-Stimulated Response Element (ISRE), so that the cell line is designed to monitor the JAK/STAT signaling pathway activity. Functional activity of the cell line has been validated by IFN-alpha (Figure 1).
NFAT Luciferase-RFP Reporter Lentivirus
78617-H	BPS Bioscience	500 µl x 2	835 EUR
Description: The NFAT Luciferase-RFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and RFP (Red Fluorescent Protein) cassette driven by the NFAT response element located upstream of the minimal TATA promoter and a hygromycin or puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or RFP expression. RFP fluoresces red-orange when excited; it has an excitation wavelength of 553 nm, and an emission wavelength of 574 nm.
NFAT Luciferase-RFP Reporter Lentivirus
78617-P	BPS Bioscience	500 µl x 2	835 EUR
Description: The NFAT Luciferase-RFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and RFP (Red Fluorescent Protein) cassette driven by the NFAT response element located upstream of the minimal TATA promoter and a hygromycin or puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or RFP expression. RFP fluoresces red-orange when excited; it has an excitation wavelength of 553 nm, and an emission wavelength of 574 nm.
GATA3 Luciferase Reporter-HEK293 Cell Line
RC1009	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The GATA3 Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the GATA3 response element, so that the cell line is designed to measure the transcriptional activity of GATA3. As a zinc-finger transcription factor, GATA3 (GATA-binding protein 3) plays a critical role in early and late T cell differentiation, which regulates Th1/Th2 differentiation. GATA3 has been shown to induce Th2 differentiation and repress Th1 differentiation. GATA3 is also known to promote the secretion of IL-4, IL-5 and IL-13 from Th2 cells. The GATA3 induction by phorbol 12-myristate 13-acetate (PMA)  is shown in Figure 1.
STAT1 Luciferase Reporter-RAW264.7 Cell Line
RC1013	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	4536 EUR
Description: The STAT1 Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the interferon (IFN) gamma activation sequence-based STAT1 response element, so that the cell line is designed to measure the transcriptional activity of STAT1. As a transcription factor, Signal Transducer and Activator of Transcription 1 (STAT1) is activated through phosphorylation at tyrosine 701 in response to various cytokines and growth factors such as IFN-alpha, IFN-gamma, IL-6, EGF and PDGF. The phosphorylated STAT1 forms homodimers or heterodimers with STAT3, and the dimers translocate to nucleus in which DNA binding/promoter induction occurs. The STAT1 induction by IFN-gamma is shown in Figure 1.
STAT3 Luciferase Reporter-HEK293 Cell Line
RC1014	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	4536 EUR
Description: The STAT3 Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter under the transcriptional control of the STAT3 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT3. As a transcription factor, Signal Transducer and Activator of Transcription 3 (STAT3) is activated through phosphorylation at tyrosine 705 in response to various cytokines including IL-6, interferons, epidermal growth factor, hepatocyte growth factor and leukemia inhibitory factor. The phosphorylated STAT3 forms homodimers or heterodimers with STAT1, and the dimers translocate to nucleus in which DNA binding/promoter induction occurs. The STAT3 induction by IL-6 is shown in Figure 1.
STAT4 Luciferase Reporter-HEK293 Cell Line
RC1040	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	4536 EUR
Description: The STAT4 Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a member of the STAT transcription factor family and plays a central role in generating inflammation during protective immune responses and immune-mediated diseases.  The STAT4 induction by interferon gamma is shown in Figure 1.
FOXP3 Luciferase Reporter-Jurkat Cell Line
RC1044	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The FOXP3 Luciferase Reporter cell line is a stably transfected Jurkat T cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Forkhead box P3 (FOXP3) promoter. As a member of the forkhead transcription factor family, FOXP3 is a key transcription factor that functions in the development and function of regulatory T cells. Functional activity of the cell line has been validated by phorbol 12-myristate 13-acetate (PMA) in the presence of ionomycin (Figure 1).
STAT3 Luciferase Reporter THP-1 Cell Line
78498	BPS Bioscience	2 vials	1900 EUR
Description: The STAT3 Luciferase Reporter THP-1 cell line is designed for monitoring the STAT3 signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.
Human p53 Luciferase Reporter Cell Line- RKO
ABC-RC0038	AcceGen	1 vial	Ask for price
Description: Human p53 Luciferase Reporter Cell Line- RKO is derived from human colon cancer, and stably express firefly luciferase reporter gene under the control of the p53 response element. This cell line is an ideal cellular model for monitoring the activation of p53 Pathway triggered by stimuli treatment,enforced gene expression and gene knockdown.
IL-2 Luciferase Reporter Jurkat cell line
60481	BPS Bioscience	2 vials	6875 EUR
Description: Human IL-2 reporter construct is stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by a human IL-2 promoter.
STAT5 Luciferase Reporter-Ba/F3 Cell Line
RC1035	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	4536 EUR
Description: The STAT5 Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT5 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT5. As a transcription factor, Signal Transducer and Activator of Transcription 5 (STAT5) is activated through phosphorylation at tyrosine 694 in response to many cytokines and growth factors including IL-2, IL-3, GM-CSF and prolactin. Aberrant STAT5 activity is closely related to a wide range of human cancers as STAT5 is often found to be constitutively phosphorylated in cancer cells. The phosphorylated STAT5 forms homodimers or heterodimers with other STATs, and the dimers translocate to nucleus in which DNA binding/promoter induction occurs. The STAT5 induction by IL-3 is shown in Figure 1.
STAT4 Luciferase Reporter-Ba/F3 Cell Line
RC1045	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	4536 EUR
Description: The STAT4 Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT4 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT4. Signal Transducer and Activator of Transcription 4 (STAT4) is a member of the STAT transcription factor family and plays a central role in generating inflammation during protective immune responses and immune-mediated diseases. The STAT4 induction by interferon gamma is shown in Figure 1.
Bald Lentiviral Pseudovirion (Luciferase Reporter)
79943	BPS Bioscience	500 µl x 2	875 EUR
Description: The bald lentiviral pseudovirion was produced without envelope glycoproteins such as VSV-G or SARS-CoV-2 spike. It contains the firefly luciferase gene driven by a CMV promoter as the reporter. The bald lentiviral pseudovirion can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors._x000D_
Human p53 Luciferase Reporter Cell Line- HeLa
ABC-RC0037	AcceGen	1 vial	Ask for price
Description: Human p53 Luciferase Reporter Cell Line- HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of the p53 response element. This cell line is an ideal cellular model for monitoring the activation of p53 Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
IL-2 Promoter Luciferase Reporter Lentivirus
79825	BPS Bioscience	500 µl x 2	795 EUR
Description: The IL-2 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the human IL-2 promoter. After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity._x000D_
IL-8 Promoter Luciferase Reporter Lentivirus
79827	BPS Bioscience	500 µl x 2	795 EUR
Description: The IL-8 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the human IL-8 promoter. After transduction, activation of the IL-8 signaling pathway in the target cells can be monitored by measuring the luciferase activity._x000D_
GIPR/CRE Luciferase Reporter HEK293 Cell Line
78589	BPS Bioscience	2 vials	10105 EUR
Description: Recombinant HEK293 cells expressing the firefly luciferase gene under the control of cAMP response element (CRE), and with forced expression of human GIPR (Gastric Inhibitory Polypeptide receptor; NM_000164.4). Activation of GIPR in these cells can be monitored by measuring luciferase activity._x000D_The functionality of the GIPR/CRE Luciferase Reporter HEK293 Cell Line was validated in a dose-response assay using agonists gastric inhibitory peptide (GIP) and tirzepatide hydrochloride. These agonists induce luciferase activity in a dose-dependent manner as depicted in Figure 1._x000D_ _x000D_Figure 1. Illustration of the GIPR/CRE Luciferase Reporter HEK293 Cell line.
Human GR Luciferase Reporter Cell Line-HeLa
ABC-RC0014	AcceGen	1 vial	Ask for price
Description: Human GR Luciferase Reporter Cell Line-HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of GR response element. This cell line is an ideal cellular model for monitoring the activation of Glucocorticoid Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human IRF Luciferase Reporter Cell Line- HepG2
ABC-RC0021	AcceGen	1 vial	Ask for price
Description: Human IRF Luciferase Reporter Cell Line- HepG2 is derived from Human Liver Cancer, and stably express firefly luciferase reporter gene under the control of IRF response element. This cell line is an ideal cellular model for monitoring the activation of Immune Response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human NFAT Luciferase Reporter Cell Line- HeLa
ABC-RC0023	AcceGen	1 vial	Ask for price
Description: Human NFAT Luciferase Reporter Cell Line- HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of NFAT response element. This cell line is an ideal cellular model for monitoring the activation of Calcium Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
IL-8 Luciferase Reporter-RAW264.7 Cell Line
RC1001	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The IL-8 Luciferase Reporter cell line is a stably transfected RAW 264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the IL-8 promoter. IL-8 is one of the key proinflammatory chemokines or cytokines, which is produced by macrophages and other epithelial cells. Induction of IL-8 is associated with inflammation. The IL-8 induction by Toll-like receptor 4 (TLR4) ligand, LPS, is shown in Figure 1.
AP-1 Luciferase Reporter-HEK293 Cell Line
RC1002	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The AP-1 Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the activator protein 1 (AP-1). The AP-1 transcription factors are homo- or  hetero-dimers that consist of proteins belonging to a group of structurally and functionally related members of the Jun family (c-Jun, JunB and JunD), the Fos (c-Fos, FosB, Fra-1 and Fra-2) and the subfamilies of ATF (ATFa, ATF-2 and ATF-3) and JDP (JDP-1 and JDP-2).  The AP-1 induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figure 1.
NFAT Luciferase Reporter Lentivirus-79579-G
79579-G	BPS Bioscience	500 µl x 2	835 EUR
Description: The NFAT Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (hygromycin, puromycin, or G418) for the selection of stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity.
NFAT Luciferase Reporter Lentivirus-79579-H
79579-H	BPS Bioscience	500 µl x 2	860 EUR
Description: The NFAT Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter and an antibiotic selection gene (hygromycin or puromycin) for the selection of stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity.
NFAT Luciferase Reporter Lentivirus-79579-P
79579-P	BPS Bioscience	500 µl x 2	860 EUR
Description: The NFAT Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter and an antibiotic selection gene (hygromycin or puromycin) for the selection of stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity.
CGRPR/CRE Luciferase Reporter HEK293 Cell Line
78325	BPS Bioscience	2 vials	10340 EUR
Description: Recombinant HEK293 cell line stably expressing full-length human Calcitonin receptor-like receptor (CALCRL/CRLR/CLR; accession number: NM_005795) and containing a firefly luciferase gene under the control of multimerized cAMP response element (CRE). This cell line can be used to measure the EC50 and IC50 of CALCRL agonists and antagonists using the luciferase reporter activity.
GR-GAL4 Luciferase Reporter Jurkat Cell Line
78525	BPS Bioscience	2 vials	2275 EUR
Description: The Glucocorticoid Receptor (GR)-GAL4 Luciferase Reporter Jurkat Cell Line contains an engineered transcription factor stably integrated into the genome of Jurkat cells, which consists of the glucocorticoid receptor ligand binding domain fused to the DNA binding domain of GAL4. This fusion construct activates firefly luciferase expression which is under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cell line is validated for response to stimulation of dexamethasone and to the treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.
Human HIF Luciferase Reporter Cell Line-Hela
ABC-RC0016	AcceGen	1 vial	Ask for price
Description: Human HIF Luciferase Reporter Cell Line-Hela is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of HIF response element. This cell line is an ideal cellular model for monitoring the activation of Hypoxia Response Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human IRF Luciferase Reporter Cell Line- HEK293
ABC-RC0020	AcceGen	1 vial	Ask for price
Description: Human IRF Luciferase Reporter Cell Line- HEK293 is derived from human embryonic kidney, and stably express firefly luciferase reporter gene under the control of IRF response element. This cell line is an ideal cellular model for monitoring the activation of Immune Response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human Stat1 Luciferase Reporter Cell Line- Hela
ABC-RC0040	AcceGen	1 vial	Ask for price
Description: Human Stat1 Luciferase Reporter Cell Line- Hela is derived from human cervical cancer,and stably express firefly luciferase reporter gene under the control of the STAT1 response element. This cell line is an ideal cellular model for monitoring the activation of JAK-STAT Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human Stat3 Luciferase Reporter Cell Line- Hela
ABC-RC0041	AcceGen	1 vial	Ask for price
Description: Human Stat3 Luciferase Reporter Cell Line- Hela is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of the STAT3 response element. This cell line is an ideal cellular model for monitoring the activation of JAK-STAT Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Mouse NFkB Luciferase Reporter Cell Line-MEF
ABC-RC0067	AcceGen	1 vial	Ask for price
Description: Mouse NFkB Luciferase Reporter Cell Line-MEF is derived from murine embryonic fibroblast,and stably express firefly luciferase reporter gene under the control of NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
NF-kB Luciferase Reporter-RAW264.7 Cell Line
RC1000	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The NF-kB Luciferase Reporter cell line is a stably transfected RAW 264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the NF-kB response element. NF-kB is a key transcription factor that is involved in immune and inflammatory responses, developmental processes, cellular growth and apoptosis.
MIP-2 Luciferase Reporter-HEK293 Cell Line
RC1003	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The MIP-2 Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the MIP-2 promoter. Macrophage inflammatory protein 2 (MIP-2) is a small cytokine that belongs to the C-X-C chemokine family and is also known as CXCL2. MIP-2 is one of the major proinflammatory cytokines, which is induced by innate immune receptors such TLRs and Nods, and also mediates LPS-induced osteoclastogenesis. The MIP-2 induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figures 1.
MIP-2 Luciferase Reporter-RAW264.7 Cell Line
RC1004	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The MIP-2 Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the MIP-2 promoter. Macrophage inflammatory protein 2 (MIP-2) is a small cytokine that belongs to the C-X-C chemokine family and is also known as CXCL2. MIP-2 is one of the major proinflammatory cytokines, which is induced by innate immune receptors such TLRs and Nods, and also mediates LPS-induced osteoclastogenesis. The MIP-2 induction by Toll-like receptor 4 (TLR4) ligand, LPS, is shown in Figure 1.
TCF/LEF Luciferase Reporter-HEK293 Cell Line
RC1019	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The TCF/LEF Luciferase Reporter cell line is a stably transfected HEK293 cell line which expresses Renilla luciferase reporter gene under the control of the TCF/LEF response element. This cell line is designed to monitor the transcriptional activity of TCF/LEF and can be used for studying Wnt signaling pathways as well as screening of activators or inhibitors that affect the TCF/LEF transcriptional activity.
NF-kB Luciferase Reporter-HEK293 Cell Line
RC1025	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The NF-kB Luciferase Reporter cell line is a stably transfected HEK293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the NF-kB response element. NF-kB is a key transcription factor that is involved in immune and inflammatory responses, developmental processes, cellular growth and apoptosis. The NF-kB induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figure 1.
NF-kB Luciferase Reporter-Jurkat Cell Line
RC1042	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The NF-kB Luciferase Reporter cell line is a stably transfected Jurkat T cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the NF-kB response element. NF-kB is a key transcription factor that is involved in immune and inflammatory responses, developmental processes, cellular growth and apoptosis. The NF-kB induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figure 1.
XRE Luciferase Reporter Lentivirus (AhR Signaling)
78672	BPS Bioscience	500 µl x 2	835 EUR
Description: The Xenobiotic response element (XRE) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by three copies of an XRE located upstream of the minimal TATA promoter (Figure 1), and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the activation of aryl hydrocarbon receptor (AhR) in the target cells can be monitored by measuring the luciferase activity.
ADAR1 Dual Luciferase Reporter HEK293 Cell Line
78547	BPS Bioscience	2 vials	19950 EUR
Description: The ADAR1 Luciferase Reporter HEK293 cell line is designed to monitor RNA editing by Adenosine deaminase acting on RNA (ADAR1). This cell line stably expresses ADAR1 under the control of a CMV promoter and a separate ADAR editing reporter construct expressed under the control of another CMV promoter. The reporter contains the gene encoding firefly luciferase, which is constitutively expressed in the cells, upstream of the gene encoding the GluA2 ADAR substrate followed by the Renilla luciferase gene. The sequence corresponding to GluA2 has been modified to contain an amber stop codon (UAG). When edited by ADAR, this stop codon (UAG) will be changed to UIG (A to I edit), which is read as tryptophan (UGG) by the translation machinery. This edit allows translation to occur all the way to the end of the reporter mRNA and results in the expression of Renilla luciferase. Conversely, in the absence of ADAR1 activity, translation terminates at the stop codon and Renilla is not expressed. Reporter activity is read out as the Renilla Luciferase/Firefly luciferase ratio whereby inhibition of ADAR activity, and thus the UAG (stop) to UGG (tryptophan) conversion rate, will result in a dose-dependent decrease in the Renilla luciferase/Firefly luciferase ratio.
Human FXR Luciferase Reporter Cell Line-HepG2
ABC-RC0013	AcceGen	1 vial	Ask for price
Description: Farnesoid X receptor (FXR, NR1H4) is a member of the nuclear hormone receptor superfamily. These nuclear hormone receptors are ligand-activated transcription factors that elicit their actions by binding to hormone response elements (HREs) in the promoters of target genes and regulating transcription in response to lipophilic ligands.Gentaur now offers Human FXR Luciferase Reporter Cell Line-HepG2 to the research community. This high-quality stable cell lines will facilitate further molecular studies of FXR pathway its functions.
Human NFkB Luciferase Reporter Cell Line-MCF7
ABC-RC0031	AcceGen	1 vial	Ask for price
Description: Human NFkB Luciferase Reporter Cell Line-MCF7 is derived from human breast cancer, and stably express firefly luciferase reporter gene under the control of the NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human NFκB Luciferase Reporter Cell Line-HeLa
ABC-RC0033	AcceGen	1 vial	Ask for price
Description: NFκB Luciferase Reporter Cell Line-HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of the NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human NFkB Luciferase Reporter Cell Line-A549
ABC-KH16937	AcceGen	1 vial vial	Ask for price
Description: Human NFkB Luciferase Reporter Cell line is derived from human lung cancer,and stably express firefly luciferase reporter gene under the control of the NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
SRF-RE Luciferase Reporter-HEK293 Cell Line
RC1033	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	2772 EUR
Description: The SRF-RE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the serum response factor-response element (SRF-RE). The SRF-RE reporter cell line can be used for studying GPCR-linked RhoA signaling pathways as well as screening of agonists, antagonists or signaling inhibitors related with the RhoA signaling pathways. Functional activity of the cell line has been validated by serum stimulation assay (Figure 1).
NFAT Reporter (Luciferase) - THP-1 Cell Line
78320	BPS Bioscience	2 vials	1810 EUR
Description: The NFAT reporter (Luciferase)-THP-1 cell line is designed for monitoring the NFAT (nuclear factor of activated T-cells) signaling pathway in THP-1 cells by measuring luciferase activity. It contains a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter. Upon activation by NFAT activators such as Ionomycin, endogenous NFAT transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.
IL-6 Luciferase Reporter-NIH 3T3 Cell Line
RC1016	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The IL-6 Luciferase Reporter cell line is a stably transfected NIH 3T3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the IL-6 promoter. As a pleiotropic cytokine, interleukin 6 (IL-6) has pro- and anti-inflammatory roles which is not only involved in normal functions of the immune system, hematopoiesis and  metabolism but also involved in the pathogenesis of metabolic and cardiovascular diseases. IL-6 gene induction is generally regulated by several transcription factors that activate the consensus sequences in the IL-6 promoter region, which include AP-1, C/EBP-beta and NF-kB in response to various proinflammatory cytokines, growth factors, and pathogen-associated molecular patterns such as Toll-like receptor (TLR) ligands. The IL-6 induction by lipopolysaccharide (LPS), the TLR4 ligand, is shown in Figure 1.
TMIGD2/NFAT Luciferase Reporter Jurkat Cell Line
78323	BPS Bioscience	2 vials	10340 EUR
Description: Recombinant Jurkat cell line expressing firefly luciferase under the control of an NFAT response element, and with constitutive expression of human TMIGD2 (Transmembrane and immunoglobulin domain containing 2; CD28H; NM_144615). Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity.
IL-15 Responsive Luciferase Reporter Cell Line
78402	BPS Bioscience	2 vials	10175 EUR
Description: This recombinant Jurkat cell line is a biologically relevant system to measure activation of the IL-15 cytokine receptor by IL-15. The cells were engineered for constitutive expression of human CD122 (IL-15Rβ; IL-2Rβ; NM_000878.4), and conditional expression of firefly luciferase driven by STAT5 response elements located upstream of the minimal TATA promoter. Expression of CD122 allows formation of a functional IL-15 receptor at the surface of Jurkat cells, which naturally express high levels of CD132 (also known as IL-15 receptor subunit γc). Activation of the STAT5 signaling pathway in response to IL-15 or IL-15 analogs can be monitored by measuring luciferase activity.
Human MRF Luciferase Reporter Cell Line-HEK293
ABC-RC0022	AcceGen	1 vial	Ask for price
Description: Human MRF Luciferase Reporter Cell Line-HEK293 is derived from human embryonic Kidney, and stably express firefly luciferase reporter gene under the control of MRF response element. This cell line is an ideal cellular model for monitoring the activation of Metal response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human NFkB Luciferase Reporter Cell Line-HepG2
ABC-RC0029	AcceGen	1 vial	Ask for price
Description: Human NFkB Luciferase Reporter Cell Line-HepG2 is derived from human liver cancer, and stably express firefly luciferase reporter gene under the control of the NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Mouse HIF Luciferase Reporter Cell Line-NIH3T3
ABC-RC0065	AcceGen	1 vial	Ask for price
Description: Mouse HIF Luciferase Reporter Cell Line-NIH3T3 is derived from mouse fibroblast, and stably express firefly luciferase reporter gene under the control of the HIF response element. This cell line is an ideal cellular model for monitoring the activation of Hypoxia Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human SBE Luciferase Reporter Cell Line-HEK293
ABC-RC0121	AcceGen	1 vial	Ask for price
Description: The SBE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the smad binding element (SBE). SMADs are intracellular signaling mediators that transduce extracellular signals from transforming growth factor beta (TGF-beta) ligands to the nucleus where they activate downstream gene transcription. The TGF-beta signaling pathway is involved in many cellular processes in both the adult organism and the developing embryo including cell growth, cell differentiation, apoptosis, cellular homeostasis and other cellular functions.
CD16-NFAT Luciferase Reporter Jurkat Cell Line
T6504	ABM	1x10^6 cells / 1.0 ml	Ask for price
TLR3/IFNB Luciferase Reporter-HEK293 Cell Line
RC1022	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	6552 EUR
Description: The TLR3/IFNB Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses full-length human Toll-like receptor 3 (TLR3) and Renilla luciferase reporter gene under the transcriptional control of the human IFN-beta (IFNB) promoter. Functional activity of the cell line has been validated by TLR3 ligand assay (Figure 1), in which upon activation by poly (I:C), TLR3 quickly initiates TRIF-dependent signaling pathway and mediates nuclear translocation of IRF3 that induces IFNB promoter activity.The TLR3/IFNB activation by poly (I:C) is shown in Figure 1.
TLR3/ISRE Luciferase Reporter-HEK293 Cell Line
RC1023	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	6552 EUR
Description: The TLR3/ISRE Luciferase cell line is a stably transfected HEK 293 cell line which expresses full-length human Toll-like receptor 3 (TLR3) and Renilla luciferase reporter gene under the transcriptional control of the interferon stimulated response element (ISRE). Functional activity of the cell line has been validated by TLR3 ligand assay (Figure 1), in which upon activation by poly (I:C), TLR3 quickly initiates TRIF-dependent signaling pathway and mediates nuclear translocation of IRF3 that induces ISRE activity.The TLR3/ISRE activation by poly (I:C) is shown in Figure 1.
Human CREB Luciferase Reporter Cell Line-HEK293
ABC-RC0008	AcceGen	1 vial	Ask for price
Description: Human CREB Luciferase Reporter Cell Line-HEK293 is derived from human embryonic kidney, and stably express firefly luciferase reporter gene under the control of CREB response element. This cell line is an ideal cellular model for monitoring the activation of cAMP,PKA,CaMK Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human CREB Luciferase Reporter Cell Line-NIH3T3
ABC-RC0009	AcceGen	1 vial	Ask for price
Description: Human CREB Luciferase Reporter Cell Line-NIH3T3 is derived from mouse fibroblast,and stably express firefly luciferase reporter gene under the control of CREB response element. This cell line is an ideal cellular model for monitoring the activation of cAMP, PKA, CaMK Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human HIF Luciferase Reporter Cell Line-Neuro2a
ABC-RC0017	AcceGen	1 vial	Ask for price
Description: Human HIF Luciferase Reporter Cell Line-Neuro2a is derived from mouse neuroblastoma, and stably express firefly luciferase reporter gene under the control of HIF response element. This cell line is an ideal cellular model for monitoring the activation of Hypoxia receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human NFkB Luciferase Reporter Cell Line-HEK293
ABC-RC0028	AcceGen	1 vial	Ask for price
Description: Human NFkB Luciferase Reporter Cell Line-HEK293 is derived from human embryonic kidney, and stably express firefly luciferase reporter gene under the control of the NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.The Human NFKB reporter (luc) cell line-HEK293 from Gentaur is designed for screening inhibitors of NF-κB and for monitoring NF-κB signaling pathway activity. It contains a firefly luciferase gene driven by four copies of NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
TNF-beta Luciferase Reporter-HEK293 Cell Line
RC1007	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The TNF-beta Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the TNF-beta promoter. Tumor necrosis factor-beta (TNF-beta) one of the major proinflammatory cytokines, which is produced predominantly by mitogen-stimulated T lymphocytes and leukocytes. TNF-beta is involved in the regulation of various biological processes such as cell proliferation, differentiation and apoptosis. TNF-beta gene polymorphisms are also significantly associated with disease susceptibility and phenotype in patients with psoriatic arthritis. The TNF-beta induction by phorbol 12-myristate 13-acetate (PMA)  is shown in Figure 1.
TNF-beta Luciferase Reporter-RAW264.7 Cell Line
RC1008	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The TNF-beta Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the TNF-beta promoter. Tumor necrosis factor-beta (TNF-beta) one of the major proinflammatory cytokines, which is produced predominantly by mitogen-stimulated T lymphocytes and leukocytes. TNF-beta is involved in the regulation of various biological processes such as cell proliferation, differentiation and apoptosis. TNF-beta gene polymorphisms are also significantly associated with disease susceptibility and phenotype in patients with psoriatic arthritis. The TNF-beta induction by lipopolysaccharide (LPS), the Toll-like receptor 4 (TLR4) ligand, is shown in Figure 1.
Human NFAT Luciferase Reporter Cell Line- NIH 3T3
ABC-RC0025	AcceGen	1 vial	Ask for price
Description: Human NFAT Luciferase Reporter Cell Line- NIH 3T3 is derived from mouse fibroblast, and stably express firefly luciferase reporter gene under the control of NFAT response element. This cell line is an ideal cellular model for monitoring the activation of Calcium Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human TCF/LEF Luciferase Reporter Cell Line- HeLa
ABC-RC0043	AcceGen	1 vial	Ask for price
Description: Human TCF/LEF Luciferase Reporter Cell Line- HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of TCF/LEF response element. This cell line is an ideal cellular model for monitoring the activation of Wnt/b-catenin Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Mouse NFkB Luciferase Reporter Cell Line- NIH 3T3
ABC-RC0066	AcceGen	1 vial	Ask for price
Description: Mouse NFkB Luciferase Reporter Cell Line- NIH 3T3 is derived from mouse fibroblast,and stably express firefly luciferase reporter gene under the control of the NFkB Response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
TLR4/IL-8 Luciferase Reporter-HeLa Cell Line
RC1024	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	6552 EUR
Description: The TLR4/IL-8 Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses human TLR4, MD-2 and CD14 as well Renilla luciferase reporter gene under the transcriptional control of the IL-8 promoter. IL-8 is one of the major pro-inflammatory cytokines induced by ligand (such as LPS)-mediated Toll-like receptor 4 (TLR4) activation. TLR4 is one of the key innate immune receptors, which is activated by LPS and can lead to sepsis upon dysregulation. The TLR4/IL-8 activation by LPS is shown in Figure 1.
Mouse NFkB Luciferase Reporter Cell Line-Neuro2a
ABC-RC0068	AcceGen	1 vial	Ask for price
Description: Mouse NFkB Luciferase Reporter Cell Line-Neuro2a is derived from mouse neuroblastoma, and stably express firefly luciferase reporter gene under the control of NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
TNF-alpha Luciferase Reporter-HEK293 Cell Line
RC1005	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The TNF-alpha Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the TNF-alpha promoter. Tumor necrosis factor-alpha (TNF-alpha) is one of the major proinflammatory cytokines and can induce systemic inflammation, apoptotic cell death, sepsis and cachexia. Dysregulation of TNF-alpha induction is often involved in various human diseases including inflammatory bowel disease, cancer and Alzheimer's disease.   The TNF-alpha induction by phorbol 12-myristate 13-acetate (PMA)  is shown in Figure 1.
TNF-alpha Luciferase Reporter-RAW264.7 Cell Line
RC1006	BosterBio	1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.	1864.8 EUR
Description: The TNF-alpha Luciferase Reporter cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the TNF-alpha promoter. Tumor necrosis factor-alpha (TNF-alpha) is one of the major proinflammatory cytokines and can induce systemic inflammation, apoptotic cell death, sepsis and cachexia. Dysregulation of TNF-alpha induction is often involved in various human diseases including inflammatory bowel disease, cancer and Alzheimer's disease. The TNF-alpha induction by lipopolysaccharide (LPS), the Toll-like receptor 4 (TLR4) ligand, is shown in Figure 1.
Human AP-1 Luciferase Reporter Cell Line-HeLa
ABC-RC0004	AcceGen	1 vial	Ask for price
Description: Human AP-1 Luciferase Reporter Cell Line-HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of AP-1 response element. This cell line is an ideal cellular model for monitoring the activation of JNK, ERK, MAPK Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human STAT6 Luciferase Reporter Cell Line-Ba/F3
ABC-RC029L	AcceGen	1 vial	Ask for price
Description: The human STAT6 Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT6 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT6. Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the STAT transcription factor family and plays an important role in adaptive immunity by transducing signals from extracellular cytokines including IL-4.
AZ-GR Stable HeLa Luciferase Reporter Cell Line
T3103	ABM	1x10^6 cells / 1.0 ml	3950 EUR
Human NFAT Luciferase Reporter Cell Line- Jurkat T
ABC-RC0024	AcceGen	1 vial	Ask for price
Description: Human NFAT Luciferase Reporter Cell Line- Jurkat T is derived from human T lymphocyte,and stably express firefly luciferase reporter gene under the control of NFAT response element. This cell line is an ideal cellular model for monitoring the activation of Calcium Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Human TCF/LEF Luciferase Reporter Cell Line- HEK293
ABC-RC0042	AcceGen	1 vial	Ask for price
Description: Human TCF/LEF Luciferase Reporter Cell Line- HEK293 is derived from human embryonic kidney, and stably express firefly luciferase reporter gene under the control of the TCF/LEF response element. This cell line is an ideal cellular model for monitoring the activation of Wnt/b-catenin Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.
Monkey HIF Luciferase Reporter Cell Line-Cos-7
ABC-RC0064	AcceGen	1 vial	Ask for price
Description: Monkey HIF Luciferase Reporter Cell Line-Cos-7 was established by transfection using a pTA-HIF-luciferase reporter vector, which contains 4 repeats of HIF binding sites, a minimal promoter upstream of the firefly luciferase coding region, along with hygromycin expression vector followed by hygromycin selection. The hygromycin resistant clones were subsequently screened for CoCl2-induced luciferase activity.
PZ-TR Stable HepG2 Luciferase Reporter Cell Line
T3177	ABM	1x10^6 cells / 1.0 ml	3950 EUR
SuperLight Luciferase Repo0er Gene Assay Kit
SLLU-01K	BioAssay Systems	1000	469 EUR
SuperLight Luciferase Repo0er Gene Assay Kit
SLLU-200	BioAssay Systems	200	179 EUR

These genomes could be observed in retinal cells within 3 hours of AAV subroeting delivery, were about full length and correlated with a vector expression in photoreceptors and retinal pigment epithelium. Saber-fish has easily detected AAV genomes in the liver and muscles according to retro-orbital and intramuscular AAV injections, which respectively demonstrate its utility in different tissues. Using Saber-Fish, we also noted that retinal microglia, a type of cell deemed refractory to AAV transduction, is actually infected by several AAV serotypes, but seem to degrade AAV genomes before nuclear location. Our results show that Saber-Fish can be used to visualize in situ AAV genomes, allowing studies on AAV vector biology and tracking cells transduced after vector administration.