Over the last 25 years, a swine vesicular disease (SVD) has occurred in Italy mainly subclinic. Therefore, regular tests of faecal samples from suspected assets and high rolling premises were fundamental to identifying the circulation of the virus and performing SVD eradication. In this study, we evaluated the diagnostic performance of six genomic amplification methods, using positive fecal samples of 78 different epidemics (1997-2014), which included different lines. Comparison of three RT-PCRS, designed to amplify the same part of 154 NT of the 3D gene, demonstrated that a conventional and real-time test based on the Sybr green detection test showed the highest diagnostic sensitivity, detecting All samples, while real-time Taqman-based test failed three cases due to two incompatibles in the target sequence of the probe.
The specificities of diagnosis and analysis were optimal because 300 negative field samples and other enteroviruses reacted negative. Three other tests evaluated, previously described, were an inverse isothermal amplification on the 3D reverse transcriptase (Lamp RT) and two real-time RT-PCR targeting on the region of 5’UTR. Here, the presence of multiple imbalances in the probe and the primers reduced the diagnostic performance and two of the analyzes could not detect viruses of a sub-lineage. These results emphasize that the choice of tests using fewer nucleotide objectives has contributed considerably to the success of the SVD eradication plan. Acanthamoeba is a living amoeba of extended genetic diversity. This can cause infectious keratitis (IK), which can also be caused by bacteria, fungi and viruses. A high diagnostic sensitivity is essential for establishing early diagnosis of the keratitis associated with Acanthamoeba. Here we have studied the applicability of new generation sequencing (NGS) – detection of ribosomal genes and the differentiation of ribosomal genes (16s-18) (16s-18) with respect to a specific real-time PCR for detection of 'Acanthamoeba Two hundred dna corneal rappings extracts and projected by Acanthamoeba-specific. Real-time PCR has been analyzed using an internal NGS dosage.
The joint regression model (JRM) is used to describe the changes in the trend in many applications and relies on the detection of participation points (changePoints). However, the methods of detecting existing join points, namely the search methods of the grid (GS) – are demanding in a manner comprising and, consequently, the maximum number of calculable join points is limited. In this document, we have developed a sealing model of the genetic algorithm (GAJP) in which an explicitly decoupled computer procedure for optimization and regression is used to integrate a binary genetic algorithm into the JRM for optimal detection point of participation. The combinations of participation points were represented in the form of binary chromosomes and genetic operations were carried out to determine the optimal solution by minimizing the Fitness function, the Bayesian information criterion (BIC) and BIC3.
New genome sequence detection via natural vector convex hull method
It remains difficult to find existing but undiscovered genome sequence mutations or predicting the potential mutations of genome sequence based on actual sequence data. Motivated by this, we develop approaches to detect new non-discovered genome sequences. Because the discovery of new genome sequences through biological experiments is with high resource intensity, we want to achieve the new genome sequence detection task mathematically.
However, little literature tells us how to detect new non-discovered genome sequence mutations mathematically. We form a new frame based on a natural vector convex hull method that performs an alignment-free sequence analysis. Our newly developed approaches, random algorithm permutation with penalty (rap) and random algorithm permutation with sanction and costrising search (RAPCOS), use the properties of geometry captured by natural vectors. In our experience, we discover a genome sequence of mathematically human immunodeficiency of human immunodeficiency using certain real HIV genome sequences. Significantly, the proposed methods are applicable to the resolution of the new genome sequence detection challenge and many good properties, such as robustness, fast convergence and rapid calculation.
In situ detection of adeno-associated viral vector genomes with fish-fish
Gene therapy with recombinant inviolized virus vectors (AAV) is a promising modality for the treatment of various human diseases. Nevertheless, there are still important gaps in our understanding of the biology of AAV vectors, because in part to the lack of robust methods to follow the Capsides and genomes AAV. In this study, we describe a new application of the amplification of the fluorescence exchange of the in situ hybridization fluorescence exchange (saber-fish) which allowed the visualization and quantification of individual AAV genomes after the administration of Vector in the mouse.
SuperLight Dual Luciferase Reporter Gene Assay Kit |
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| SLDL-100 | BioAssay Systems | 100 | 240 EUR |
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Description: Bioluminescent reagent system allows rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells within 10 min. Kit size: 100 tests. Shelf life: 12 months. Shipping: ambient temp; storage: -20°C. |
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Luciferase Reporter Assay Kit |
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| 55R-1540 | Fitzgerald | 200 assays | 294 EUR |
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Description: Assay Kit for detection of Luciferase Reporter in the research laboratory |
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Luciferase Reporter Assay Kit |
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| K2181-200 | ApexBio | 200 assays | 217.2 EUR |
Luciferase Reporter Assay Kit |
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| K801-200 | Biovision | each | 235.2 EUR |
Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow* |
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| 12518 | AAT Bioquest | 1 plate | 211.2 EUR |
Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow* |
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| 12519 | AAT Bioquest | 10 plates | 471.6 EUR |
Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow* |
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| 12520 | AAT Bioquest | 100 plates | 3139.2 EUR |
Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow* |
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| 12530 | AAT Bioquest | 1 plate | 262.8 EUR |
Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow* |
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| 12531 | AAT Bioquest | 10 Plates | 842.4 EUR |
Amplite™ Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow* |
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| 12532 | AAT Bioquest | 100 plates | 4183.2 EUR |
Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow* |
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| 12535 | AAT Bioquest | 1 plate | 262.8 EUR |
Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow* |
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| 12536 | AAT Bioquest | 10 plates | 1051.2 EUR |
Amplite™ Renilla Luciferase Reporter Gene Assay Kit *Bright Glow* |
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| 12537 | AAT Bioquest | 100 plates | 4183.2 EUR |
Dual Luciferase Reporter Assay Kit |
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| DL101-01 | Vazyme | 100 rxn | 309.6 EUR |
Single-Luciferase Reporter Assay Kit |
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| 20-abx098133 | Abbexa |
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Double-Luciferase Reporter Assay Kit |
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| 20-abx098134 | Abbexa |
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Single-Luciferase (Renilla) Reporter Assay Kit |
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| 20-abx298011 | Abbexa |
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RARα Reporter Cellular Assay Pack |
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| 79322 | BPS Bioscience | 2 vials | 2390 EUR |
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Description: The RARα Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor alpha (RARα). The pack contains the RARα Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (GenBank Accession No. NM_000964). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARα is confirmed by Western blotting._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. |
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RARβ Reporter Cellular Assay Pack |
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| 79323 | BPS Bioscience | 2 vials | 2050 EUR |
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Description: The RARβ Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor beta (RARβ). The pack contains the RARβ Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (GenBank Accession No. P10826-2). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARβ is confirmed by Western blotting._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. |
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RARγ Reporter Cellular Assay Pack |
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| 79324 | BPS Bioscience | 2 vials | 2050 EUR |
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Description: The RARγ Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of retinoic acid receptor gamma (RARγ). The pack contains the RARγ Reporter (Luc)-HEK293 Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (GenBank Accession No. P13631-1). This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid, and the expression of RARγ is confirmed by Western blotting._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 6) that has been optimized for use with HEK293 cells. Thaw Medium 6 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. |
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PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
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| PIN320A-KIT | SBI | 1 Kit | 5929.2 EUR |
PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
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| PIN340iPS-KIT | SBI | 1 Kit | 5929.2 EUR |
NF-κB Reporter Cellular Assay Pack (CHOK1) |
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| 79325 | BPS Bioscience | 2 vials | 1245 EUR |
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Description: The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc)- CHO-K1 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine. _x000D_Additionally, the pack includes cell culture medium (Thaw Medium 3) that has been optimized for use with CHO-K1 cells. Thaw Medium 3 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. |
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NF-κB Reporter Cellular Assay Pack (HCT116) |
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| 79326 | BPS Bioscience | 2 vials | 1245 EUR |
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Description: The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc)- HCT-116 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 7) that has been optimized for use with HCT-116 cells. Thaw Medium 7 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D__x000D_ |
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NF-κB Reporter Cellular Assay Pack (HEK293) |
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| 79327 | BPS Bioscience | 2 vials | 1515 EUR |
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Description: The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc)-HEK293 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 1) that has been optimized for use with HEK293 cells. Thaw Medium 1 includes 10% fetal bovine serum, non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D_ |
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TFEB promoter-luciferase reporter |
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| PVT18227 | Lifescience Market | 2 ug | 360 EUR |
SRE Luciferase Reporter Lentivirus |
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| 78627 | BPS Bioscience | 500 µl x 2 | 835 EUR |
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Description: The SRE (Serum Response Element) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the Serum Response Element located upstream of the minimal TATA promoter . After transduction, activation of the MAPK/ERK signaling pathway in the target cells can be monitored by measuring the luciferase activity. |
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Myc Luciferase Reporter Lentivirus |
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| 78628 | BPS Bioscience | 500 µl x 2 | 835 EUR |
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Description: The Myc Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the Myc response element located upstream of the minimal TATA promoter and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the Myc signaling pathway in the target cells can be monitored by measuring the luciferase activity. |
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UAS Luciferase Reporter Lentivirus |
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| 78631 | BPS Bioscience | 500 µl x 2 | 835 EUR |
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Description: The UAS (Upstream Activation Sequence) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by a multimerized GAL4 upstream activation sequence (UAS) located upstream of the minimal TATA promoter and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the UAS-controlled signaling pathway in the target cells can be monitored by measuring the luciferase activity. |
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p53 Luciferase Reporter Lentivirus |
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| 78666 | BPS Bioscience | 500 µl x 2 | 835 EUR |
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Description: The p53 Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by p53 response elements located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, p53-regulated gene expression in the target cells can be monitored by measuring the luciferase activity. |
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HRE Luciferase Reporter Lentivirus |
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| 78668 | BPS Bioscience | 500 µl x 2 | 835 EUR |
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Description: The Hypoxia Response Element (HRE) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by four copies of a hypoxia response elements (HRE) located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the induction of hypoxia in the target cells can be monitored by measuring the luciferase activity. |
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STAT3 Luciferase Reporter Lentivirus |
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| 79744 | BPS Bioscience | 500 µl x 2 | 860 EUR |
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Description: The STAT3 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of STAT3-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the STAT3 signaling pathway in the target cells can be monitored by measuring the luciferase activity._x000D_ |
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STAT5 Luciferase Reporter Lentivirus |
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| 79745 | BPS Bioscience | 500 µl x 2 | 835 EUR |
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Description: The STAT5 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of STAT5-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the STAT5 signaling pathway in the target cells can be monitored by measuring the luciferase activity. |
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TEAD Luciferase Reporter Lentivirus |
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| 79833 | BPS Bioscience | 500 µl x 2 | 875 EUR |
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Description: The Hippo pathway regulates cell proliferation and cell death. It is activated by high cell density and cell stress to stop cell proliferation and induce apoptosis. The mammalian Hippo pathway comprises MST kinases and LATS kinases. When the Hippo pathway is activated, MST kinases phosphorylate LATS kinases, which phosphorylate transcriptional co-activators YAP and TAZ. Unphosphorylated YAP and TAZ remain in nucleus and interact with TEAD/TEF transcriptional factors to turn on cell cycle-promoting gene transcription. However, when phosphorylated, YAP and TAZ are recruited from the nucleus to the cytosol, so that the YAP and TAZ-dependent gene transcription is turned off. Dysfunction of the Hippo pathway is frequently detected in human cancer and its down-regulation correlates with the aggressive properties of cancer cells and poor prognosis. The TEAD Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the TEAD response elements located upstream of the minimal TATA promoter. After transduction, activation of the Hippo pathway in the target cells can be monitored by measuring the luciferase activity._x000D_ |
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ARE Luciferase Reporter Lentivirus |
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| 79869 | BPS Bioscience | 500 µl x 2 | 875 EUR |
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Description: The Nrf2 antioxidant response pathway plays an important role in the cellular antioxidant defense. Nrf2, a basic leucine zipper transcription factor, induces the expression of antioxidant and phase II enzymes by binding to the ARE (antioxidant response element) region of the gene promoter. Under basal conditions, Nrf2 is retained in the cytosol by binding to the cytoskeletal protein Keap1. Upon exposure to oxidative stress or other ARE activators, Nrf2 is released from Keap1 and translocates to the nucleus, where it can bind to the ARE, leading to the expression of antioxidant and phase II enzymes that protect the cell from oxidative damage. The ARE Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by ARE located upstream of the minimal TATA promoter. After transduction, activation of the Nrf2 antioxidant response pathway in the target cells can be monitored by measuring the luciferase activity. |
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AlleleBalanced Luciferase Assay Kit |
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| ABP-PA-ABLA001 | Allele Biotech | 100 RXNS | Ask for price |
AlleleBalanced Luciferase Assay Kit |
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| ABP-PA-ABLA011 | Allele Biotech | 1000 RXNS | Ask for price |
AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
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| GE624A-KIT | SBI | 1 kit | 2558.4 EUR |
NFAT Luciferase-RFP Reporter Lentivirus |
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| 78617-H | BPS Bioscience | 500 µl x 2 | 835 EUR |
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Description: The NFAT Luciferase-RFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and RFP (Red Fluorescent Protein) cassette driven by the NFAT response element located upstream of the minimal TATA promoter and a hygromycin or puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or RFP expression. RFP fluoresces red-orange when excited; it has an excitation wavelength of 553 nm, and an emission wavelength of 574 nm. |
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NFAT Luciferase-RFP Reporter Lentivirus |
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| 78617-P | BPS Bioscience | 500 µl x 2 | 835 EUR |
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Description: The NFAT Luciferase-RFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and RFP (Red Fluorescent Protein) cassette driven by the NFAT response element located upstream of the minimal TATA promoter and a hygromycin or puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or RFP expression. RFP fluoresces red-orange when excited; it has an excitation wavelength of 553 nm, and an emission wavelength of 574 nm. |
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NF-κB Luciferase Reporter Lentivirus |
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| 79564 | BPS Bioscience | 500 µl x 2 | 875 EUR |
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Description: The NF-κB Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After transduction, activation of the NF-κB signaling pathway in the target cells can be monitored by measuring the luciferase activity. |
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CRE/CREB Luciferase Reporter Lentivirus |
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| 79580 | BPS Bioscience | 500 µl x 2 | 835 EUR |
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Description: The main role of the cAMP response element, or CRE, is mediating the effects of Protein Kinase A (PKA) by way of transcription. Upon phosphorylation, CREB forms a functionally active dimer that binds the CRE element within the promoters of target genes and activates transcription. CRE is at the focus of many extracellular and intracellular signaling pathways, including cAMP, calcium, GPCR (G-protein coupled receptors) and neurotrophins. The cAMP/PKA signaling pathway is critical to numerous life processes in living organisms.The CRE/CREB Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized cAMP response element (CRE) located upstream of the minimal TATA promoter. After transduction, activation of the cAMP/PKA signaling pathway in the target cells can be monitored by measuring the luciferase activity. |
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Bald Lentiviral Pseudovirion (Luciferase Reporter) |
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| 79943 | BPS Bioscience | 500 µl x 2 | 875 EUR |
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Description: The bald lentiviral pseudovirion was produced without envelope glycoproteins such as VSV-G or SARS-CoV-2 spike. It contains the firefly luciferase gene driven by a CMV promoter as the reporter. The bald lentiviral pseudovirion can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors._x000D_ |
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Firefly Luciferase Assay Kit (Lyophilized) |
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| 30075-1 | Biotium | 150TST | 150 EUR |
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Description: Minimum order quantity: 1 unit of 150TST |
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Firefly Luciferase Assay Kit (Lyophilized) |
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| 30075-2 | Biotium | 1000TST | 550.8 EUR |
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Description: Minimum order quantity: 1 unit of 1000TST |
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Renilla Luciferase Assay Kit 2.0 |
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| 30082-1 | Biotium | 150AS | 206.4 EUR |
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Description: Minimum order quantity: 1 unit of 150AS |
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Renilla Luciferase Assay Kit 2.0 |
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| 30082-2 | Biotium | 1000AS | 712.8 EUR |
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Description: Minimum order quantity: 1 unit of 1000AS |
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Renilla Luciferase Assay Kit 2.0 |
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| 30082-T | Biotium | 50AS | 136.8 EUR |
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Description: Minimum order quantity: 1 unit of 50AS |
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Firefly Luciferase Assay Kit 2.0 |
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| 30085-1 | Biotium | 150AS | 150 EUR |
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Description: Minimum order quantity: 1 unit of 150AS |
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Firefly Luciferase Assay Kit 2.0 |
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| 30085-2 | Biotium | 1000AS | 550.8 EUR |
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Description: Minimum order quantity: 1 unit of 1000AS |
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Firefly Luciferase Assay Kit 2.0 |
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| 30085-T | Biotium | 50AS | 130.8 EUR |
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Description: Minimum order quantity: 1 unit of 50AS |
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LucyQ Firefly Luciferase Assay Kit |
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| L0010-010 | GenDepot | 100 Assays | 327.6 EUR |
LucyQ Firefly Luciferase Assay Kit |
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| L0010-100 | GenDepot | 1000 Assays | 740.4 EUR |
LucyQ Gaussia Luciferase Assay Kit |
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| L0011-010 | GenDepot | 100 Assays | 346.8 EUR |
LucyQ Gaussia Luciferase Assay Kit |
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| L0011-100 | GenDepot | 1000 Assays | 1132.8 EUR |
LucyQ Renilla Luciferase Assay Kit |
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| L0012-010 | GenDepot | 100 Assays | 327.6 EUR |
LucyQ Renilla Luciferase Assay Kit |
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| L0012-100 | GenDepot | 1000 Assays | 740.4 EUR |
Bald VSV Delta G (Luciferase Reporter) |
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| 78636-1 | BPS Bioscience | 100 µl | 395 EUR |
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Description: The bald VSV Delta G (Luciferase Reporter) was produced without envelope glycoproteins. It contains the firefly luciferase gene as the reporter. The bald VSV Delta G (Luciferase Reporter) can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors. |
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Bald VSV Delta G (Luciferase Reporter) |
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| 78636-2 | BPS Bioscience | 500 µl x 2 | 1995 EUR |
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Description: The bald VSV Delta G (Luciferase Reporter) was produced without envelope glycoproteins. It contains the firefly luciferase gene as the reporter. The bald VSV Delta G (Luciferase Reporter) can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors. |
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XRE Luciferase Reporter Lentivirus (AhR Signaling) |
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| 78672 | BPS Bioscience | 500 µl x 2 | 835 EUR |
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Description: The Xenobiotic response element (XRE) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by three copies of an XRE located upstream of the minimal TATA promoter (Figure 1), and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the activation of aryl hydrocarbon receptor (AhR) in the target cells can be monitored by measuring the luciferase activity. |
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NFAT Luciferase Reporter Lentivirus-79579-G |
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| 79579-G | BPS Bioscience | 500 µl x 2 | 835 EUR |
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Description: The NFAT Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (hygromycin, puromycin, or G418) for the selection of stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity. |
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NFAT Luciferase Reporter Lentivirus-79579-H |
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| 79579-H | BPS Bioscience | 500 µl x 2 | 860 EUR |
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Description: The NFAT Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter and an antibiotic selection gene (hygromycin or puromycin) for the selection of stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity. |
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NFAT Luciferase Reporter Lentivirus-79579-P |
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| 79579-P | BPS Bioscience | 500 µl x 2 | 860 EUR |
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Description: The NFAT Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter and an antibiotic selection gene (hygromycin or puromycin) for the selection of stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity. |
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IL-2 Promoter Luciferase Reporter Lentivirus |
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| 79825 | BPS Bioscience | 500 µl x 2 | 795 EUR |
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Description: The IL-2 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the human IL-2 promoter. After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity._x000D_ |
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IL-8 Promoter Luciferase Reporter Lentivirus |
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| 79827 | BPS Bioscience | 500 µl x 2 | 795 EUR |
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Description: The IL-8 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the human IL-8 promoter. After transduction, activation of the IL-8 signaling pathway in the target cells can be monitored by measuring the luciferase activity._x000D_ |
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RNA/DNA Purification Kit (Magnetic Bead) |
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| DA0623 | Daan Gene | 32 test/kit | Ask for price |
PCR detection kit for Monkeypox Virus |
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| DA1430 | Daan Gene | 24 test/kit | 129.6 EUR |
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Description: Monkeypox is a viral zoonosis (a virus transmitted to humans from animals) with symptoms very similar to those seen in the past in smallpox patients, although it is clinically less severe. It is caused by the monkeypox virus which belongs to the orthopoxvirus genus of the Poxviridae family. There are two clades of monkeypox virus: the West African clade and the Congo Basin (Central African) clade. The name monkeypox originates from the initial discovery of the virus in monkeys in a Danish laboratory in 1958. The first human case was identified in a child in the Democratic Republic of the Congo in 1970. |
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CLOuD9 Gene Expression Regulation Kit (includes 10 ug each of dCas9-PYL1 and dCas9-ABI1 lentivectors, and 100 ul of 0.5M Inducer Agent) |
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| CASCL9-100A-KIT | SBI | 1 Kit | 1358.4 EUR |
Frit Kit |
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| FRIT-KIT | Next Advance | 1each | 148.8 EUR |
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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
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Column Packing Kit |
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| PACK-KIT | Next Advance | 1pack | 1242 EUR |
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
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PCR Mycoplasma Detection Kit |
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| M034-Kit | TOKU-E | Kit | 319.2 EUR |
IL-2 Luciferase Reporter Jurkat cell line |
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| 60481 | BPS Bioscience | 2 vials | 6875 EUR |
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Description: Human IL-2 reporter construct is stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by a human IL-2 promoter. |
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NFAT Reporter (Luciferase) - THP-1 Cell Line |
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| 78320 | BPS Bioscience | 2 vials | 1810 EUR |
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Description: The NFAT reporter (Luciferase)-THP-1 cell line is designed for monitoring the NFAT (nuclear factor of activated T-cells) signaling pathway in THP-1 cells by measuring luciferase activity. It contains a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter. Upon activation by NFAT activators such as Ionomycin, endogenous NFAT transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. |
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TMIGD2/NFAT Luciferase Reporter Jurkat Cell Line |
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| 78323 | BPS Bioscience | 2 vials | 10340 EUR |
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Description: Recombinant Jurkat cell line expressing firefly luciferase under the control of an NFAT response element, and with constitutive expression of human TMIGD2 (Transmembrane and immunoglobulin domain containing 2; CD28H; NM_144615). Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity. |
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CGRPR/CRE Luciferase Reporter HEK293 Cell Line |
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| 78325 | BPS Bioscience | 2 vials | 10340 EUR |
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Description: Recombinant HEK293 cell line stably expressing full-length human Calcitonin receptor-like receptor (CALCRL/CRLR/CLR; accession number: NM_005795) and containing a firefly luciferase gene under the control of multimerized cAMP response element (CRE). This cell line can be used to measure the EC50 and IC50 of CALCRL agonists and antagonists using the luciferase reporter activity. |
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IL-15 Responsive Luciferase Reporter Cell Line |
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| 78402 | BPS Bioscience | 2 vials | 10175 EUR |
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Description: This recombinant Jurkat cell line is a biologically relevant system to measure activation of the IL-15 cytokine receptor by IL-15. The cells were engineered for constitutive expression of human CD122 (IL-15Rβ; IL-2Rβ; NM_000878.4), and conditional expression of firefly luciferase driven by STAT5 response elements located upstream of the minimal TATA promoter. Expression of CD122 allows formation of a functional IL-15 receptor at the surface of Jurkat cells, which naturally express high levels of CD132 (also known as IL-15 receptor subunit γc). Activation of the STAT5 signaling pathway in response to IL-15 or IL-15 analogs can be monitored by measuring luciferase activity. |
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STAT3 Luciferase Reporter THP-1 Cell Line |
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| 78498 | BPS Bioscience | 2 vials | 1900 EUR |
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Description: The STAT3 Luciferase Reporter THP-1 cell line is designed for monitoring the STAT3 signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene. |
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GR-GAL4 Luciferase Reporter Jurkat Cell Line |
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| 78525 | BPS Bioscience | 2 vials | 2275 EUR |
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Description: The Glucocorticoid Receptor (GR)-GAL4 Luciferase Reporter Jurkat Cell Line contains an engineered transcription factor stably integrated into the genome of Jurkat cells, which consists of the glucocorticoid receptor ligand binding domain fused to the DNA binding domain of GAL4. This fusion construct activates firefly luciferase expression which is under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cell line is validated for response to stimulation of dexamethasone and to the treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway. |
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ADAR1 Dual Luciferase Reporter HEK293 Cell Line |
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| 78547 | BPS Bioscience | 2 vials | 19950 EUR |
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Description: The ADAR1 Luciferase Reporter HEK293 cell line is designed to monitor RNA editing by Adenosine deaminase acting on RNA (ADAR1). This cell line stably expresses ADAR1 under the control of a CMV promoter and a separate ADAR editing reporter construct expressed under the control of another CMV promoter. The reporter contains the gene encoding firefly luciferase, which is constitutively expressed in the cells, upstream of the gene encoding the GluA2 ADAR substrate followed by the Renilla luciferase gene. The sequence corresponding to GluA2 has been modified to contain an amber stop codon (UAG). When edited by ADAR, this stop codon (UAG) will be changed to UIG (A to I edit), which is read as tryptophan (UGG) by the translation machinery. This edit allows translation to occur all the way to the end of the reporter mRNA and results in the expression of Renilla luciferase. Conversely, in the absence of ADAR1 activity, translation terminates at the stop codon and Renilla is not expressed. Reporter activity is read out as the Renilla Luciferase/Firefly luciferase ratio whereby inhibition of ADAR activity, and thus the UAG (stop) to UGG (tryptophan) conversion rate, will result in a dose-dependent decrease in the Renilla luciferase/Firefly luciferase ratio. |
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GIPR/CRE Luciferase Reporter HEK293 Cell Line |
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| 78589 | BPS Bioscience | 2 vials | 10105 EUR |
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Description: Recombinant HEK293 cells expressing the firefly luciferase gene under the control of cAMP response element (CRE), and with forced expression of human GIPR (Gastric Inhibitory Polypeptide receptor; NM_000164.4). Activation of GIPR in these cells can be monitored by measuring luciferase activity._x000D_The functionality of the GIPR/CRE Luciferase Reporter HEK293 Cell Line was validated in a dose-response assay using agonists gastric inhibitory peptide (GIP) and tirzepatide hydrochloride. These agonists induce luciferase activity in a dose-dependent manner as depicted in Figure 1._x000D_
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NFAT Luciferase-eGFP Reporter Lentivirus-78656-G |
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| 78656-G | BPS Bioscience | 500 µl x 2 | 835 EUR |
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Description: The NFAT Luciferase-eGFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and eGFP cassette driven by the NFAT response element located upstream of the minimal TATA promoter (Figure 1) and a puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or eGFP expression. |
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NFAT Luciferase-eGFP Reporter Lentivirus-78656-P |
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| 78656-P | BPS Bioscience | 500 µl x 2 | 835 EUR |
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Description: The NFAT Luciferase-eGFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and eGFP cassette driven by the NFAT response element located upstream of the minimal TATA promoter (Figure 1) and a puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or eGFP expression. |
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NFAT Luciferase-eGFP Reporter Jurkat Cell Line |
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| 78662 | BPS Bioscience | 2 vials | 2195 EUR |
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Description: Recombinant Jurkat T cells expressing both firefly luciferase and enhanced GFP (eGFP) under the control of NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway can be monitored by examining either firefly luciferase or eGFP expression. |
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SBE Luciferase Reporter Lentivirus (TGFβ/SMAD Pathway) |
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| 79806 | BPS Bioscience | 500 µl x 2 | 875 EUR |
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Description: The SBE Luciferase Reporter Lentivirus (TGFβ/SMAD signaling pathway) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized SBE-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the TGFβ/SMAD signaling pathway can be monitored by measuring the luciferase activity._x000D_ |
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AP1 Luciferase Reporter Lentivirus (JNK Signaling Pathway) |
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| 79823 | BPS Bioscience | 500 µl x 2 | 795 EUR |
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Description: The stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) family of proteins includes mitogen-activated protein kinases (MAPKs) that are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun. c-Jun then binds to the activator protein-1 (AP1) response element and induces AP1 transcription. The AP1 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by AP1 response element located upstream of the minimal TATA promoter. After transduction, activation of the JNK signaling pathway and AP1 mediated activity in the target cells can be monitored by measuring the luciferase activity. |
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AP1 Reporter Kit (JNK Pathway) |
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| 60612 | BPS Bioscience | 500 rxns. | 565 EUR |
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Description: The AP1 Reporter Kit is designed for monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured cells. The kit contains a transfection-ready AP1 luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized AP1 responsive elements located upstream of a minimal promoter. The AP1 reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity. |
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Notch1 Pathway Reporter Kit (Human) |
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| 79503 | BPS Bioscience | 500 rxns. | 650 EUR |
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Description: Notch Pathway Reporter kit is designed for monitoring the activity of the Notch signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for Human NOTCH1 that has a deletion of the entire extracellular domain, leaving the transmembrane and intracellular domains intact (Notch1DE). Inside the cells, the NOTCH1 DE is cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency._x000D_The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity._x000D_This kit contains the expression vector for Human Notch1DE. We also offer the Mouse Notch1DE expression vector (BPS Bioscience #60509), as well as a Mouse Notch1 NICD expression vector, which is constitutively active without processing by γ-secretase (BPS Bioscience #79504)._x000D_ |
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Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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| CAS400A-KIT | SBI | 1 kit (10 rxn) | 1332 EUR |
Pesticide Kit Containing All 10 Multi-Compound Mixes |
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| SPXPR-KIT | Scientific Laboratory Supplies | 1ML | 1923.18 EUR |
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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| CAS410A-KIT | SBI | 1 kit (10 rxn) | 798 EUR |
Cas9-EGFP Protein and T7 gRNA SmartNuclease Synthesis Kit |
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| CAS420A-KIT | SBI | 1 kit (10 rxn) | 828 EUR |
CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV100PA-KIT | SBI | 1 Kit | 1358.4 EUR |
CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV105PA-KIT | SBI | 1 Kit | 1358.4 EUR |
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV120PA-KIT | SBI | 1 Kit | 1358.4 EUR |
MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV125PA-KIT | SBI | 1 Kit | 1358.4 EUR |
2C::tdTomato Reporter |
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| PVT10473 | Lifescience Market | 2 ug | 319.2 EUR |
pMIR- Reporter Plasmid |
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| PVT1324 | Lifescience Market | 2 ug | 319.2 EUR |
Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS700A-KIT | SBI | 10 rxn | 1358.4 EUR |
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS720A-KIT | SBI | 10 rxn | 1358.4 EUR |
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS740A-KIT | SBI | 10 rxn | 1358.4 EUR |
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
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| CAS510A-KIT | SBI | 1 Kit | 966 EUR |
Firefly & Renilla Luciferase Single Tube Assay Kit |
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| 30081-1 | Biotium | 150AS | 292.8 EUR |
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Description: Minimum order quantity: 1 unit of 150AS |
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Firefly & Renilla Luciferase Single Tube Assay Kit |
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| 30081-2 | Biotium | 1000AS | 1180.8 EUR |
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Description: Minimum order quantity: 1 unit of 1000AS |
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Firefly & Renilla Luciferase Single Tube Assay Kit |
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| 30081-T | Biotium | 50AS | 186 EUR |
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Description: Minimum order quantity: 1 unit of 50AS |
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LucyQ Duo-Luciferase(Firefly & Renilla) Assay Kit |
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| L0013-010 | GenDepot | 100 Assays | 380.4 EUR |
LucyQ Duo-Luciferase(Firefly & Renilla) Assay Kit |
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| L0013-100 | GenDepot | 1000 Assays | 2217.6 EUR |
Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV200PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV205PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV220PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV225PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Minicircle Single Reporter: CMV-Luciferase Minicircle (30 ug) |
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| BLIV510MC-1 | SBI | 30 ug | 859.2 EUR |
Minicircle Single Reporter: EF1 Luciferase DNA (30 ug) |
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| BLIV511MC-1 | SBI | 30 ug | 859.2 EUR |
Hippo Pathway TEAD Luciferase Reporter MCF7 cell line |
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| 60618 | BPS Bioscience | 2 vials | 2445 EUR |
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Description: The TEAD Reporter - MCF7 cell line contains the firefly luciferase gene under the control of TEAD responsive elements stably integrated into the human breast cancer cell line, MCF7. Inside the cells, basal unphosphorylated YAP/TAZ remains in the nucleus and induces the constitutive expression of luciferase reporter. The cell line is validated for the inhibition of the expression of luciferase reporter by the activators of the Hippo pathway. |
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NF-κB-Luciferase Reporter (Luc) - Jurkat Cell Line |
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| 60651 | BPS Bioscience | 2 vials | 2340 EUR |
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Description: NF-κB luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene. |
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KIR3DL3/IL-2 Luciferase Reporter Jurkat Cell Line |
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| 78322 | BPS Bioscience | 2 vials | 10855 EUR |
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Description: Recombinant Jurkat cell line expressing firefly luciferase under the control of an IL-2-responsive promoter, and with constitutive expression of human KIR3DL3 (Killer Cell Immunoglobulin Like Receptor, Three Ig Domains and Long Cytoplasmic Tail 3; GenBank accession #BC143802.1 corresponding to KIR3DL3*00402 allele). HHLA2 (B7-H7) mediates an immune-stimulatory signal via TMIGD2 (Transmembrane and immunoglobulin domain containing 2; CD28H) in naïve T cells while it delivers an immune-inhibitory signal through KIR3DL3 in activated T cells and Natural Killer (NK) cells. |
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B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line |
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| 78363 | BPS Bioscience | 2 vials | 11095 EUR |
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Description: B2M (Beta-2-Microglobulin) has been genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells. Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity. |
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TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line |
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| 78556 | BPS Bioscience | 2 vials | 12205 EUR |
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Description: This cell line is a knockout of TCR (T Cell Receptor). The TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from recombinant Jurkat cells stably expressing the firefly luciferase gene under the control of NFAT response elements.This cell line has been functionally validated and does not respond to anti-CD3 agonist antibodies, as opposed to parental NFAT-Luciferase Reporter Jurkat cells (BPS Bioscience #60621). |
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VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) |
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| 78634-1 | BPS Bioscience | 100 µl | 795 EUR |
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Description: The VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) was produced by re-expression of VSV-G as the envelope glycoprotein using the VSV Delta G system in which VSV-G is deleted. The pseudovirions contain the firefly luciferase gene; therefore, the VSV-G mediated cell entry can be measured via luciferase activity. The VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) can be used as a positive control of transduction for other VSV pseudotypes containing the envelope glycoproteins of heterologous viruses in a Biosafety Level 2 facility. |
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VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) |
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| 78634-2 | BPS Bioscience | 500 µl x 2 | 3995 EUR |
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Description: The VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) was produced by re-expression of VSV-G as the envelope glycoprotein using the VSV Delta G system in which VSV-G is deleted. The pseudovirions contain the firefly luciferase gene; therefore, the VSV-G mediated cell entry can be measured via luciferase activity. The VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) can be used as a positive control of transduction for other VSV pseudotypes containing the envelope glycoproteins of heterologous viruses in a Biosafety Level 2 facility. |
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CD160/NFAT - Luciferase Reporter - Jurkat Recombinant Cell Line |
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| 79594 | BPS Bioscience | 2 vials | 9400 EUR |
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Description: Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human CD160. CD160 is a GPIanchored glycoprotein member of the Ig superfamily, also known as BY55, NK1, and NK28. GenBank Accession # NM_007053._x000D__x000D_ |
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LILRB4 NFAT-Luciferase Reporter Jurkat Recombinant Cell Line |
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| 79750 | BPS Bioscience | 2 vials | 9870 EUR |
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Description: Recombinant Jurkat cells constitutively expressing human LILRB4 (Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 4, GenBank Accession #NM_001278426) and the firefly luciferase gene under the control of NFAT response elements. |
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ISRE Luciferase Reporter Lentivirus (JAK/STAT Signaling Pathway) |
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| 79824 | BPS Bioscience | 500 µl x 2 | 820 EUR |
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Description: The JAK/STAT pathway is activated by various cytokines and growth factors and plays a critical role in cell growth, hematopoiesis, and immune response. In mammals, there are four JAKs (JAK1, JAK2, JAK3 and TYK2) and seven STAT proteins. IFNα is a Type I interferon. Binding of IFNα to its receptor leads to the activation of JAK1 and TYK2, which in turn phosphorylate and activate STAT1 and STAT2. The phosphorylated STAT1 and 2 form a heterodimer and bind to IRF9/p48, forming a protein complex ISGF3. This complex translocates to the nucleus and binds to the ISRE (Interferon Stimulated Response Element) in the promoter region, thereby promoting transcription of interferon-inducible genes. The ISRE Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized ISRE response element located upstream of the minimal TATA promoter. After transduction, the activity of Type I interferon-induced JAK/STAT signaling pathway in the target cells can be monitored by measuring the luciferase activity. |
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Spike (SARS-CoV-2) Pseudotyped Lentivirus (Luciferase Reporter) |
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| 79942-1 | BPS Bioscience | 100 µl | 875 EUR |
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Description: The SARS-CoV-2 Spike Pseudotyped Lentivirus were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1) as the envelope glycoproteins instead of the commonly used VSV-G. These pseudovirions also contain the firefly luciferase gene driven by a CMV promoter, therefore, the spike-mediated cell entry can be conveniently measured via luciferase reporter activity. The SARS-CoV-2 Spike pseudotyped lentivirus can be used to measure the activity of neutralizing antibody against SARS-CoV-2 in a Biosafety Level 2 facility._x000D_ _x000D_ |
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These genomes could be observed in retinal cells within 3 hours of AAV subroeting delivery, were about full length and correlated with a vector expression in photoreceptors and retinal pigment epithelium. Saber-fish has easily detected AAV genomes in the liver and muscles according to retro-orbital and intramuscular AAV injections, which respectively demonstrate its utility in different tissues. Using Saber-Fish, we also noted that retinal microglia, a type of cell deemed refractory to AAV transduction, is actually infected by several AAV serotypes, but seem to degrade AAV genomes before nuclear location. Our results show that Saber-Fish can be used to visualize in situ AAV genomes, allowing studies on AAV vector biology and tracking cells transduced after vector administration.