Diagnostic Performances of Different Genome Amplification Assays for the Detection of Swine Vesicular Disease Virus in Relation to Genomic Lineages That Circulated in Italy

Over the last 25 years, a swine vesicular disease (SVD) has occurred in Italy mainly subclinic. Therefore, regular tests of faecal samples from suspected assets and high rolling premises were fundamental to identifying the circulation of the virus and performing SVD eradication. In this study, we evaluated the diagnostic performance of six genomic amplification methods, using positive fecal samples of 78 different epidemics (1997-2014), which included different lines. Comparison of three RT-PCRS, designed to amplify the same part of 154 NT of the 3D gene, demonstrated that a conventional and real-time test based on the Sybr green detection test showed the highest diagnostic sensitivity, detecting All samples, while real-time Taqman-based test failed three cases due to two incompatibles in the target sequence of the probe.

The specificities of diagnosis and analysis were optimal because 300 negative field samples and other enteroviruses reacted negative. Three other tests evaluated, previously described, were an inverse isothermal amplification on the 3D reverse transcriptase (Lamp RT) and two real-time RT-PCR targeting on the region of 5’UTR. Here, the presence of multiple imbalances in the probe and the primers reduced the diagnostic performance and two of the analyzes could not detect viruses of a sub-lineage. These results emphasize that the choice of tests using fewer nucleotide objectives has contributed considerably to the success of the SVD eradication plan. Acanthamoeba is a living amoeba of extended genetic diversity. This can cause infectious keratitis (IK), which can also be caused by bacteria, fungi and viruses. A high diagnostic sensitivity is essential for establishing early diagnosis of the keratitis associated with Acanthamoeba. Here we have studied the applicability of new generation sequencing (NGS) – detection of ribosomal genes and the differentiation of ribosomal genes (16s-18) (16s-18) with respect to a specific real-time PCR for detection of 'Acanthamoeba Two hundred dna corneal rappings extracts and projected by Acanthamoeba-specific. Real-time PCR has been analyzed using an internal NGS dosage.

The joint regression model (JRM) is used to describe the changes in the trend in many applications and relies on the detection of participation points (changePoints). However, the methods of detecting existing join points, namely the search methods of the grid (GS) – are demanding in a manner comprising and, consequently, the maximum number of calculable join points is limited. In this document, we have developed a sealing model of the genetic algorithm (GAJP) in which an explicitly decoupled computer procedure for optimization and regression is used to integrate a binary genetic algorithm into the JRM for optimal detection point of participation. The combinations of participation points were represented in the form of binary chromosomes and genetic operations were carried out to determine the optimal solution by minimizing the Fitness function, the Bayesian information criterion (BIC) and BIC3.

New genome sequence detection via natural vector convex hull method

It remains difficult to find existing but undiscovered genome sequence mutations or predicting the potential mutations of genome sequence based on actual sequence data. Motivated by this, we develop approaches to detect new non-discovered genome sequences. Because the discovery of new genome sequences through biological experiments is with high resource intensity, we want to achieve the new genome sequence detection task mathematically.

However, little literature tells us how to detect new non-discovered genome sequence mutations mathematically. We form a new frame based on a natural vector convex hull method that performs an alignment-free sequence analysis. Our newly developed approaches, random algorithm permutation with penalty (rap) and random algorithm permutation with sanction and costrising search (RAPCOS), use the properties of geometry captured by natural vectors. In our experience, we discover a genome sequence of mathematically human immunodeficiency of human immunodeficiency using certain real HIV genome sequences. Significantly, the proposed methods are applicable to the resolution of the new genome sequence detection challenge and many good properties, such as robustness, fast convergence and rapid calculation.

Diagnostic Performances of Different Genome Amplification Assays for the Detection of Swine Vesicular Disease Virus in Relation to Genomic Lineages That Circulated in Italy
Diagnostic Performances of Different Genome Amplification Assays for the Detection of Swine Vesicular Disease Virus in Relation to Genomic Lineages That Circulated in Italy

 

In situ detection of adeno-associated viral vector genomes with fish-fish

Gene therapy with recombinant inviolized virus vectors (AAV) is a promising modality for the treatment of various human diseases. Nevertheless, there are still important gaps in our understanding of the biology of AAV vectors, because in part to the lack of robust methods to follow the Capsides and genomes AAV. In this study, we describe a new application of the amplification of the fluorescence exchange of the in situ hybridization fluorescence exchange (saber-fish) which allowed the visualization and quantification of individual AAV genomes after the administration of Vector in the mouse.

SuperLight™ Dual Luciferase Reporter Gene Assay Kit

SLDL-100 BioAssay Systems 100 189 EUR
Description: Bioluminescent reagent system for rapid quantitation of firelfy and Ranilla luciferase reporter gene expression in transfected cells. Key Features: High sensitivity and wide detection range. Detection of as little of 2 fg luciferase. Compatible with routine laboratory and HTS formats. Assays can be performed in tubes or microplates, and measured with any luminometer. Can be readily automated on HTS liquid handling systems. Fast and convenient. Three step assay allows detection of dual luciferase levels within 20 minutes. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 2 fg luciferase.

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

12518 AAT Bioquest 1 plate 166 EUR

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

12519 AAT Bioquest 10 plates 446 EUR

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

12520 AAT Bioquest 100 plates 3321 EUR

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

12518-1plate AAT Bioquest 1 plate 166 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

12519-10plates AAT Bioquest 10 plates 446 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

12520-100plates AAT Bioquest 100 plates 3321 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12530 AAT Bioquest 1 plate 222 EUR

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12531 AAT Bioquest 10 Plates 846 EUR

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12532 AAT Bioquest 100 plates 4447 EUR

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12535 AAT Bioquest 1 plate 222 EUR

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12536 AAT Bioquest 10 plates 1070 EUR

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12537 AAT Bioquest 100 plates 4447 EUR

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12530-1plate AAT Bioquest 1 plate 222 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12531-10Plates AAT Bioquest 10 Plates 846 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

12532-100plates AAT Bioquest 100 plates 4447 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12535-1plate AAT Bioquest 1 plate 222 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12536-10plates AAT Bioquest 10 plates 1070 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

12537-100plates AAT Bioquest 100 plates 4447 EUR
Description: Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

Luciferase Reporter Assay Kit

55R-1540 Fitzgerald 200 assays 294 EUR
Description: Assay Kit for detection of Luciferase Reporter in the research laboratory

Luciferase Reporter Assay Kit

K801-200 Biovision each 235.2 EUR

Luciferase Reporter Assay Kit

K2181-200 ApexBio 200 assays 217.2 EUR

Dual Luciferase Reporter Assay Kit

DL101-01 Vazyme 100 rxn 309.6 EUR

ARE Luciferase Reporter Lentivirus

79869 BPS Bioscience 500 µl x 2 875 EUR
Description: The Nrf2 antioxidant response pathway plays an important role in the cellular antioxidant defense. Nrf2, a basic leucine zipper transcription factor, induces the expression of antioxidant and phase II enzymes by binding to the ARE (antioxidant response element) region of the gene promoter. Under basal conditions, Nrf2 is retained in the cytosol by binding to the cytoskeletal protein Keap1. Upon exposure to oxidative stress or other ARE activators, Nrf2 is released from Keap1 and translocates to the nucleus, where it can bind to the ARE, leading to the expression of antioxidant and phase II enzymes that protect the cell from oxidative damage.
The ARE Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by ARE located upstream of the minimal TATA promoter. After transduction, activation of the Nrf2 antioxidant response pathway in the target cells can be monitored by measuring the luciferase activity.

SRE Luciferase Reporter Lentivirus

78627 BPS Bioscience 500 µl x 2 835 EUR
Description: The SRE (Serum Response Element) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the Serum Response Element located upstream of the minimal TATA promoter . After transduction, activation of the MAPK/ERK signaling pathway in the target cells can be monitored by measuring the luciferase activity.

Myc Luciferase Reporter Lentivirus

78628 BPS Bioscience 500 µl x 2 835 EUR
Description: The Myc Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the Myc response element located upstream of the minimal TATA promoter and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the Myc signaling pathway in the target cells can be monitored by measuring the luciferase activity.

UAS Luciferase Reporter Lentivirus

78631 BPS Bioscience 500 µl x 2 835 EUR
Description: The UAS (Upstream Activation Sequence) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by a multimerized GAL4 upstream activation sequence (UAS) located upstream of the minimal TATA promoter and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the UAS-controlled signaling pathway in the target cells can be monitored by measuring the luciferase activity.

p53 Luciferase Reporter Lentivirus

78666 BPS Bioscience 500 µl x 2 835 EUR
Description: The p53 Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by p53 response elements located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, p53-regulated gene expression in the target cells can be monitored by measuring the luciferase activity.

HRE Luciferase Reporter Lentivirus

78668 BPS Bioscience 500 µl x 2 835 EUR
Description: The Hypoxia Response Element (HRE) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by four copies of a hypoxia response elements (HRE) located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the induction of hypoxia in the target cells can be monitored by measuring the luciferase activity.

TEAD Luciferase Reporter Lentivirus

79833 BPS Bioscience 500 µl x 2 875 EUR
Description: The Hippo pathway regulates cell proliferation and cell death. It is activated by high cell density and cell stress to stop cell proliferation and induce apoptosis. The mammalian Hippo pathway comprises MST kinases and LATS kinases. When the Hippo pathway is activated, MST kinases phosphorylate LATS kinases, which phosphorylate transcriptional co-activators YAP and TAZ. Unphosphorylated YAP and TAZ remain in nucleus and interact with TEAD/TEF transcriptional factors to turn on cell cycle-promoting gene transcription. However, when phosphorylated, YAP and TAZ are recruited from the nucleus to the cytosol, so that the YAP and TAZ-dependent gene transcription is turned off. Dysfunction of the Hippo pathway is frequently detected in human cancer and its down-regulation correlates with the aggressive properties of cancer cells and poor prognosis.
The TEAD Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the TEAD response elements located upstream of the minimal TATA promoter. After transduction, activation of the Hippo pathway in the target cells can be monitored by measuring the luciferase activity._x000D_

STAT3 Luciferase Reporter Lentivirus

79744 BPS Bioscience 500 µl x 2 860 EUR
Description: The STAT3 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of STAT3-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the STAT3 signaling pathway in the target cells can be monitored by measuring the luciferase activity._x000D_

STAT5 Luciferase Reporter Lentivirus

79745 BPS Bioscience 500 µl x 2 835 EUR
Description: The STAT5 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of STAT5-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the STAT5 signaling pathway in the target cells can be monitored by measuring the luciferase activity.

NF-κB Luciferase Reporter Lentivirus

79564 BPS Bioscience 500 µl x 2 875 EUR
Description: The NF-κB Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After transduction, activation of the NF-κB signaling pathway in the target cells can be monitored by measuring the luciferase activity.

Bald VSV Delta G (Luciferase Reporter)

78636-1 BPS Bioscience 100 µl 395 EUR
Description: The bald VSV Delta G (Luciferase Reporter) was produced without envelope glycoproteins. It contains the firefly luciferase gene as the reporter. The bald VSV Delta G (Luciferase Reporter) can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.

Bald VSV Delta G (Luciferase Reporter)

78636-2 BPS Bioscience 500 µl x 2 1995 EUR
Description: The bald VSV Delta G (Luciferase Reporter) was produced without envelope glycoproteins. It contains the firefly luciferase gene as the reporter. The bald VSV Delta G (Luciferase Reporter) can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.

CRE/CREB Luciferase Reporter Lentivirus

79580 BPS Bioscience 500 µl x 2 835 EUR
Description: The main role of the cAMP response element, or CRE, is mediating the effects of Protein Kinase A (PKA) by way of transcription. Upon phosphorylation, CREB forms a functionally active dimer that binds the CRE element within the promoters of target genes and activates transcription. CRE is at the focus of many extracellular and intracellular signaling pathways, including cAMP, calcium, GPCR (G-protein coupled receptors) and neurotrophins. The cAMP/PKA signaling pathway is critical to numerous life processes in living organisms.The CRE/CREB Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized cAMP response element (CRE) located upstream of the minimal TATA promoter. After transduction, activation of the cAMP/PKA signaling pathway in the target cells can be monitored by measuring the luciferase activity.

NFAT Luciferase-RFP Reporter Lentivirus

78617-H BPS Bioscience 500 µl x 2 835 EUR
Description: The NFAT Luciferase-RFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and RFP (Red Fluorescent Protein) cassette driven by the NFAT response element located upstream of the minimal TATA promoter and a hygromycin or puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or RFP expression. RFP fluoresces red-orange when excited; it has an excitation wavelength of 553 nm, and an emission wavelength of 574 nm.

NFAT Luciferase-RFP Reporter Lentivirus

78617-P BPS Bioscience 500 µl x 2 835 EUR
Description: The NFAT Luciferase-RFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and RFP (Red Fluorescent Protein) cassette driven by the NFAT response element located upstream of the minimal TATA promoter and a hygromycin or puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or RFP expression. RFP fluoresces red-orange when excited; it has an excitation wavelength of 553 nm, and an emission wavelength of 574 nm.

STAT3 Luciferase Reporter THP-1 Cell Line

78498 BPS Bioscience 2 vials 1900 EUR
Description: The STAT3 Luciferase Reporter THP-1 cell line is designed for monitoring the STAT3 signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

IL-2 Luciferase Reporter Jurkat cell line

60481 BPS Bioscience 2 vials 6875 EUR
Description: Human IL-2 reporter construct is stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by a human IL-2 promoter.

Human p53 Luciferase Reporter Cell Line- RKO

ABC-RC0038 AcceGen 1 vial Ask for price
Description: Human p53 Luciferase Reporter Cell Line- RKO is derived from human colon cancer, and stably express firefly luciferase reporter gene under the control of the p53 response element. This cell line is an ideal cellular model for monitoring the activation of p53 Pathway triggered by stimuli treatment,enforced gene expression and gene knockdown.

Human p53 Luciferase Reporter Cell Line- HeLa

ABC-RC0037 AcceGen 1 vial Ask for price
Description: Human p53 Luciferase Reporter Cell Line- HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of the p53 response element. This cell line is an ideal cellular model for monitoring the activation of p53 Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Bald Lentiviral Pseudovirion (Luciferase Reporter)

79943 BPS Bioscience 500 µl x 2 875 EUR
Description: The bald lentiviral pseudovirion was produced without envelope glycoproteins such as VSV-G or SARS-CoV-2 spike. It contains the firefly luciferase gene driven by a CMV promoter as the reporter. The bald lentiviral pseudovirion can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors._x000D_

IL-2 Promoter Luciferase Reporter Lentivirus

79825 BPS Bioscience 500 µl x 2 795 EUR
Description: The IL-2 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the human IL-2 promoter. After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity._x000D_

IL-8 Promoter Luciferase Reporter Lentivirus

79827 BPS Bioscience 500 µl x 2 795 EUR
Description: The IL-8 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the human IL-8 promoter. After transduction, activation of the IL-8 signaling pathway in the target cells can be monitored by measuring the luciferase activity._x000D_

GIPR/CRE Luciferase Reporter HEK293 Cell Line

78589 BPS Bioscience 2 vials 10105 EUR
Description: Recombinant HEK293 cells expressing the firefly luciferase gene under the control of cAMP response element (CRE), and with forced expression of human GIPR (Gastric Inhibitory Polypeptide receptor; NM_000164.4). Activation of GIPR in these cells can be monitored by measuring luciferase activity._x000D_The functionality of the GIPR/CRE Luciferase Reporter HEK293 Cell Line was validated in a dose-response assay using agonists gastric inhibitory peptide (GIP) and tirzepatide hydrochloride. These agonists induce luciferase activity in a dose-dependent manner as depicted in Figure 1._x000D_

_x000D_Figure 1. Illustration of the GIPR/CRE Luciferase Reporter HEK293 Cell line.

Human GR Luciferase Reporter Cell Line-HeLa

ABC-RC0014 AcceGen 1 vial Ask for price
Description: Human GR Luciferase Reporter Cell Line-HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of GR response element. This cell line is an ideal cellular model for monitoring the activation of Glucocorticoid Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human IRF Luciferase Reporter Cell Line- HepG2

ABC-RC0021 AcceGen 1 vial Ask for price
Description: Human IRF Luciferase Reporter Cell Line- HepG2 is derived from Human Liver Cancer, and stably express firefly luciferase reporter gene under the control of IRF response element. This cell line is an ideal cellular model for monitoring the activation of Immune Response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human NFAT Luciferase Reporter Cell Line- HeLa

ABC-RC0023 AcceGen 1 vial Ask for price
Description: Human NFAT Luciferase Reporter Cell Line- HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of NFAT response element. This cell line is an ideal cellular model for monitoring the activation of Calcium Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

NFAT Luciferase Reporter Lentivirus-79579-G

79579-G BPS Bioscience 500 µl x 2 835 EUR
Description: The NFAT Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter (Figure 1) and an antibiotic selection gene (hygromycin, puromycin, or G418) for the selection of stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity.

NFAT Luciferase Reporter Lentivirus-79579-H

79579-H BPS Bioscience 500 µl x 2 860 EUR
Description: The NFAT Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter and an antibiotic selection gene (hygromycin or puromycin) for the selection of stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity.

NFAT Luciferase Reporter Lentivirus-79579-P

79579-P BPS Bioscience 500 µl x 2 860 EUR
Description: The NFAT Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter and an antibiotic selection gene (hygromycin or puromycin) for the selection of stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity.

CGRPR/CRE Luciferase Reporter HEK293 Cell Line

78325 BPS Bioscience 2 vials 10340 EUR
Description: Recombinant HEK293 cell line stably expressing full-length human Calcitonin receptor-like receptor (CALCRL/CRLR/CLR; accession number: NM_005795) and containing a firefly luciferase gene under the control of multimerized cAMP response element (CRE). This cell line can be used to measure the EC50 and IC50 of CALCRL agonists and antagonists using the luciferase reporter activity.

GR-GAL4 Luciferase Reporter Jurkat Cell Line

78525 BPS Bioscience 2 vials 2275 EUR
Description: The Glucocorticoid Receptor (GR)-GAL4 Luciferase Reporter Jurkat Cell Line contains an engineered transcription factor stably integrated into the genome of Jurkat cells, which consists of the glucocorticoid receptor ligand binding domain fused to the DNA binding domain of GAL4. This fusion construct activates firefly luciferase expression which is under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cell line is validated for response to stimulation of dexamethasone and to the treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.

Human HIF Luciferase Reporter Cell Line-Hela

ABC-RC0016 AcceGen 1 vial Ask for price
Description: Human HIF Luciferase Reporter Cell Line-Hela is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of HIF response element. This cell line is an ideal cellular model for monitoring the activation of Hypoxia Response Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human IRF Luciferase Reporter Cell Line- HEK293

ABC-RC0020 AcceGen 1 vial Ask for price
Description: Human IRF Luciferase Reporter Cell Line- HEK293 is derived from human embryonic kidney, and stably express firefly luciferase reporter gene under the control of IRF response element. This cell line is an ideal cellular model for monitoring the activation of Immune Response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human Stat1 Luciferase Reporter Cell Line- Hela

ABC-RC0040 AcceGen 1 vial Ask for price
Description: Human Stat1 Luciferase Reporter Cell Line- Hela is derived from human cervical cancer,and stably express firefly luciferase reporter gene under the control of the STAT1 response element. This cell line is an ideal cellular model for monitoring the activation of JAK-STAT Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human Stat3 Luciferase Reporter Cell Line- Hela

ABC-RC0041 AcceGen 1 vial Ask for price
Description: Human Stat3 Luciferase Reporter Cell Line- Hela is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of the STAT3 response element. This cell line is an ideal cellular model for monitoring the activation of JAK-STAT Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Mouse NFkB Luciferase Reporter Cell Line-MEF

ABC-RC0067 AcceGen 1 vial Ask for price
Description: Mouse NFkB Luciferase Reporter Cell Line-MEF is derived from murine embryonic fibroblast,and stably express firefly luciferase reporter gene under the control of NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Single-Luciferase Reporter Assay Kit

20-abx098133 Abbexa
  • 679.20 EUR
  • 566.40 EUR
  • 200 rxns
  • 50 rxns

Double-Luciferase Reporter Assay Kit

20-abx098134 Abbexa
  • 904.80 EUR
  • 622.80 EUR
  • 200 rxns
  • 50 rxns

NFAT Reporter (Luciferase) - THP-1 Cell Line

78320 BPS Bioscience 2 vials 1810 EUR
Description: The NFAT reporter (Luciferase)-THP-1 cell line is designed for monitoring the NFAT (nuclear factor of activated T-cells) signaling pathway in THP-1 cells by measuring luciferase activity. It contains a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter. Upon activation by NFAT activators such as Ionomycin, endogenous NFAT transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

XRE Luciferase Reporter Lentivirus (AhR Signaling)

78672 BPS Bioscience 500 µl x 2 835 EUR
Description: The Xenobiotic response element (XRE) Luciferase Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by three copies of an XRE located upstream of the minimal TATA promoter (Figure 1), and an antibiotic selection gene (puromycin) for the selection of stable clones. After transduction, the activation of aryl hydrocarbon receptor (AhR) in the target cells can be monitored by measuring the luciferase activity.

ADAR1 Dual Luciferase Reporter HEK293 Cell Line

78547 BPS Bioscience 2 vials 19950 EUR
Description: The ADAR1 Luciferase Reporter HEK293 cell line is designed to monitor RNA editing by Adenosine deaminase acting on RNA (ADAR1). This cell line stably expresses ADAR1 under the control of a CMV promoter and a separate ADAR editing reporter construct expressed under the control of another CMV promoter. The reporter contains the gene encoding firefly luciferase, which is constitutively expressed in the cells, upstream of the gene encoding the GluA2 ADAR substrate followed by the Renilla luciferase gene. The sequence corresponding to GluA2 has been modified to contain an amber stop codon (UAG). When edited by ADAR, this stop codon (UAG) will be changed to UIG (A to I edit), which is read as tryptophan (UGG) by the translation machinery. This edit allows translation to occur all the way to the end of the reporter mRNA and results in the expression of Renilla luciferase. Conversely, in the absence of ADAR1 activity, translation terminates at the stop codon and Renilla is not expressed. Reporter activity is read out as the Renilla Luciferase/Firefly luciferase ratio whereby inhibition of ADAR activity, and thus the UAG (stop) to UGG (tryptophan) conversion rate, will result in a dose-dependent decrease in the Renilla luciferase/Firefly luciferase ratio.

Human FXR Luciferase Reporter Cell Line-HepG2

ABC-RC0013 AcceGen 1 vial Ask for price
Description: Farnesoid X receptor (FXR, NR1H4) is a member of the nuclear hormone receptor superfamily. These nuclear hormone receptors are ligand-activated transcription factors that elicit their actions by binding to hormone response elements (HREs) in the promoters of target genes and regulating transcription in response to lipophilic ligands.Gentaur now offers Human FXR Luciferase Reporter Cell Line-HepG2 to the research community. This high-quality stable cell lines will facilitate further molecular studies of FXR pathway its functions.

Human NFkB Luciferase Reporter Cell Line-MCF7

ABC-RC0031 AcceGen 1 vial Ask for price
Description: Human NFkB Luciferase Reporter Cell Line-MCF7 is derived from human breast cancer, and stably express firefly luciferase reporter gene under the control of the NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human NFκB Luciferase Reporter Cell Line-HeLa

ABC-RC0033 AcceGen 1 vial Ask for price
Description: NFκB Luciferase Reporter Cell Line-HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of the NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human NFkB Luciferase Reporter Cell Line-A549

ABC-KH16937 AcceGen 1 vial vial Ask for price
Description: Human NFkB Luciferase Reporter Cell line is derived from human lung cancer,and stably express firefly luciferase reporter gene under the control of the NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

TMIGD2/NFAT Luciferase Reporter Jurkat Cell Line

78323 BPS Bioscience 2 vials 10340 EUR
Description: Recombinant Jurkat cell line expressing firefly luciferase under the control of an NFAT response element, and with constitutive expression of human TMIGD2 (Transmembrane and immunoglobulin domain containing 2; CD28H; NM_144615). Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity. 

IL-15 Responsive Luciferase Reporter Cell Line

78402 BPS Bioscience 2 vials 10175 EUR
Description: This recombinant Jurkat cell line is a biologically relevant system to measure activation of the IL-15 cytokine receptor by IL-15. The cells were engineered for constitutive expression of human CD122 (IL-15Rβ; IL-2Rβ; NM_000878.4), and conditional expression of firefly luciferase driven by STAT5 response elements located upstream of the minimal TATA promoter. Expression of CD122 allows formation of a functional IL-15 receptor at the surface of Jurkat cells, which naturally express high levels of CD132 (also known as IL-15 receptor subunit γc). Activation of the STAT5 signaling pathway in response to IL-15 or IL-15 analogs can be monitored by measuring luciferase activity.

Human MRF Luciferase Reporter Cell Line-HEK293

ABC-RC0022 AcceGen 1 vial Ask for price
Description: Human MRF Luciferase Reporter Cell Line-HEK293 is derived from human embryonic Kidney, and stably express firefly luciferase reporter gene under the control of MRF response element. This cell line is an ideal cellular model for monitoring the activation of Metal response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human NFkB Luciferase Reporter Cell Line-HepG2

ABC-RC0029 AcceGen 1 vial Ask for price
Description: Human NFkB Luciferase Reporter Cell Line-HepG2 is derived from human liver cancer, and stably express firefly luciferase reporter gene under the control of the NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Mouse HIF Luciferase Reporter Cell Line-NIH3T3

ABC-RC0065 AcceGen 1 vial Ask for price
Description: Mouse HIF Luciferase Reporter Cell Line-NIH3T3 is derived from mouse fibroblast, and stably express firefly luciferase reporter gene under the control of the HIF response element. This cell line is an ideal cellular model for monitoring the activation of Hypoxia Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human SBE Luciferase Reporter Cell Line-HEK293

ABC-RC0121 AcceGen 1 vial Ask for price
Description: The SBE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the smad binding element (SBE). SMADs are intracellular signaling mediators that transduce extracellular signals from transforming growth factor beta (TGF-beta) ligands to the nucleus where they activate downstream gene transcription. The TGF-beta signaling pathway is involved in many cellular processes in both the adult organism and the developing embryo including cell growth, cell differentiation, apoptosis, cellular homeostasis and other cellular functions.

CD16-NFAT Luciferase Reporter Jurkat Cell Line

T6504 ABM 1x10^6 cells / 1.0 ml Ask for price

Human NFAT Luciferase Reporter Cell Line- NIH 3T3

ABC-RC0025 AcceGen 1 vial Ask for price
Description: Human NFAT Luciferase Reporter Cell Line- NIH 3T3 is derived from mouse fibroblast, and stably express firefly luciferase reporter gene under the control of NFAT response element. This cell line is an ideal cellular model for monitoring the activation of Calcium Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human TCF/LEF Luciferase Reporter Cell Line- HeLa

ABC-RC0043 AcceGen 1 vial Ask for price
Description: Human TCF/LEF Luciferase Reporter Cell Line- HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of TCF/LEF response element. This cell line is an ideal cellular model for monitoring the activation of Wnt/b-catenin Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Mouse NFkB Luciferase Reporter Cell Line- NIH 3T3

ABC-RC0066 AcceGen 1 vial Ask for price
Description: Mouse NFkB Luciferase Reporter Cell Line- NIH 3T3 is derived from mouse fibroblast,and stably express firefly luciferase reporter gene under the control of the NFkB Response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human CREB Luciferase Reporter Cell Line-HEK293

ABC-RC0008 AcceGen 1 vial Ask for price
Description: Human CREB Luciferase Reporter Cell Line-HEK293 is derived from human embryonic kidney, and stably express firefly luciferase reporter gene under the control of CREB response element. This cell line is an ideal cellular model for monitoring the activation of cAMP,PKA,CaMK Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human CREB Luciferase Reporter Cell Line-NIH3T3

ABC-RC0009 AcceGen 1 vial Ask for price
Description: Human CREB Luciferase Reporter Cell Line-NIH3T3 is derived from mouse fibroblast,and stably express firefly luciferase reporter gene under the control of CREB response element. This cell line is an ideal cellular model for monitoring the activation of cAMP, PKA, CaMK Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human HIF Luciferase Reporter Cell Line-Neuro2a

ABC-RC0017 AcceGen 1 vial Ask for price
Description: Human HIF Luciferase Reporter Cell Line-Neuro2a is derived from mouse neuroblastoma, and stably express firefly luciferase reporter gene under the control of HIF response element. This cell line is an ideal cellular model for monitoring the activation of Hypoxia receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human NFkB Luciferase Reporter Cell Line-HEK293

ABC-RC0028 AcceGen 1 vial Ask for price
Description: Human NFkB Luciferase Reporter Cell Line-HEK293 is derived from human embryonic kidney, and stably express firefly luciferase reporter gene under the control of the NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.The Human NFKB reporter (luc) cell line-HEK293 from Gentaur is designed for screening inhibitors of NF-κB and for monitoring NF-κB signaling pathway activity. It contains a firefly luciferase gene driven by four copies of NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.

Human AP-1 Luciferase Reporter Cell Line-HeLa

ABC-RC0004 AcceGen 1 vial Ask for price
Description: Human AP-1 Luciferase Reporter Cell Line-HeLa is derived from human cervical cancer, and stably express firefly luciferase reporter gene under the control of AP-1 response element. This cell line is an ideal cellular model for monitoring the activation of JNK, ERK, MAPK Signaling Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human STAT6 Luciferase Reporter Cell Line-Ba/F3

ABC-RC029L AcceGen 1 vial Ask for price
Description: The human STAT6 Luciferase Reporter cell line is a stably transfected Ba/F3 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the STAT6 responsive promoter, so that the cell line is designed to measure the transcriptional activity of STAT6. Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the STAT transcription factor family and plays an important role in adaptive immunity by transducing signals from extracellular cytokines including IL-4.

AZ-GR Stable HeLa Luciferase Reporter Cell Line

T3103 ABM 1x10^6 cells / 1.0 ml 3950 EUR

Mouse NFkB Luciferase Reporter Cell Line-Neuro2a

ABC-RC0068 AcceGen 1 vial Ask for price
Description: Mouse NFkB Luciferase Reporter Cell Line-Neuro2a is derived from mouse neuroblastoma, and stably express firefly luciferase reporter gene under the control of NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human NFAT Luciferase Reporter Cell Line- Jurkat T

ABC-RC0024 AcceGen 1 vial Ask for price
Description: Human NFAT Luciferase Reporter Cell Line- Jurkat T is derived from human T lymphocyte,and stably express firefly luciferase reporter gene under the control of NFAT response element. This cell line is an ideal cellular model for monitoring the activation of Calcium Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human TCF/LEF Luciferase Reporter Cell Line- HEK293

ABC-RC0042 AcceGen 1 vial Ask for price
Description: Human TCF/LEF Luciferase Reporter Cell Line- HEK293 is derived from human embryonic kidney, and stably express firefly luciferase reporter gene under the control of the TCF/LEF response element. This cell line is an ideal cellular model for monitoring the activation of Wnt/b-catenin Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Monkey HIF Luciferase Reporter Cell Line-Cos-7

ABC-RC0064 AcceGen 1 vial Ask for price
Description: Monkey HIF Luciferase Reporter Cell Line-Cos-7 was established by transfection using a pTA-HIF-luciferase reporter vector, which contains 4 repeats of HIF binding sites, a minimal promoter upstream of the firefly luciferase coding region, along with hygromycin expression vector followed by hygromycin selection. The hygromycin resistant clones were subsequently screened for CoCl2-induced luciferase activity.

PZ-TR Stable HepG2 Luciferase Reporter Cell Line

T3177 ABM 1x10^6 cells / 1.0 ml 3950 EUR

SBE Luciferase Reporter Lentivirus (TGFβ/SMAD Pathway)

79806 BPS Bioscience 500 µl x 2 875 EUR
Description: The SBE Luciferase Reporter Lentivirus (TGFβ/SMAD signaling pathway) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized SBE-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the TGFβ/SMAD signaling pathway can be monitored by measuring the luciferase activity._x000D_

KIR3DL3/IL-2 Luciferase Reporter Jurkat Cell Line

78322 BPS Bioscience 2 vials 10855 EUR
Description: Recombinant Jurkat cell line expressing firefly luciferase under the control of an IL-2-responsive promoter, and with constitutive expression of human KIR3DL3 (Killer Cell Immunoglobulin Like Receptor, Three Ig Domains and Long Cytoplasmic Tail 3; GenBank accession #BC143802.1 corresponding to KIR3DL3*00402 allele). HHLA2 (B7-H7) mediates an immune-stimulatory signal via TMIGD2 (Transmembrane and immunoglobulin domain containing 2; CD28H) in naïve T cells while it delivers an immune-inhibitory signal through KIR3DL3 in activated T cells and Natural Killer (NK) cells.

Hamster ATF6 Luciferase Reporter Cell Line- CHO-K1

ABC-RC0002 AcceGen 1 vial Ask for price
Description: Hamster ATF6 Luciferase Reporter Cell Line- CHO-K1 is derived from Chinese Hamster Ovary, and stably express firefly luciferase reporter gene under the control of ATF6 response element. This cell line is an ideal cellular model for monitoring the activation of Unfolded Protein Response, ER stress Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human NRF2/ARE Luciferase Reporter Cell Line-MCF7

ABC-RC0036 AcceGen 1 vial Ask for price
Description: Human NRF2/ARE Luciferase Reporter Cell Line-MCF7 is derived from human breast cancer, and stably express firefly luciferase reporter gene under the control of the NRF2/ARE response element. This cell line is an ideal cellular model for monitoring the activation of Antioxidant Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

AZ-AHR Stable HepG2 Luciferase Reporter Cell Line

T3102 ABM 1x10^6 cells / 1.0 ml 3950 EUR

AIZ-AR Stable 22Rv1 Luciferase Reporter Cell Line

T3104 ABM 1x10^6 cells / 1.0 ml 3950 EUR

NFAT Luciferase-eGFP Reporter Jurkat Cell Line

78662 BPS Bioscience 2 vials 2195 EUR
Description: Recombinant Jurkat T cells expressing both firefly luciferase and enhanced GFP (eGFP) under the control of NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway can be monitored by examining either firefly luciferase or eGFP expression.

VSV-G Pseudotyped VSV Delta G (Luciferase Reporter)

78634-1 BPS Bioscience 100 µl 795 EUR
Description: The VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) was produced by re-expression of VSV-G as the envelope glycoprotein using the VSV Delta G system in which VSV-G is deleted. The pseudovirions contain the firefly luciferase gene; therefore, the VSV-G mediated cell entry can be measured via luciferase activity. The VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) can be used as a positive control of transduction for other VSV pseudotypes containing the envelope glycoproteins of heterologous viruses in a Biosafety Level 2 facility.

VSV-G Pseudotyped VSV Delta G (Luciferase Reporter)

78634-2 BPS Bioscience 500 µl x 2 3995 EUR
Description: The VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) was produced by re-expression of VSV-G as the envelope glycoprotein using the VSV Delta G system in which VSV-G is deleted. The pseudovirions contain the firefly luciferase gene; therefore, the VSV-G mediated cell entry can be measured via luciferase activity. The VSV-G Pseudotyped VSV Delta G (Luciferase Reporter) can be used as a positive control of transduction for other VSV pseudotypes containing the envelope glycoproteins of heterologous viruses in a Biosafety Level 2 facility.

Human ELK-TAD Luciferase Reporter Cell Line-HeLa

ABC-RC0011 AcceGen 1 vial Ask for price
Description: Human ELK-TAD Luciferase Reporter Cell Line-HeLa is derived human cervical cancer, and stably express firefly luciferase reporter gene under the control of ELK response element. This cell line is an ideal cellular model for monitoring the activation of MAPK response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human NF-kB Luciferase Reporter Cell Line-Jurkat

ABC-RC0030 AcceGen 1 vial Ask for price
Description: Human NFkB Luciferase Reporter Cell Line-Jurkat is is derived from human T lymphocyte cells, and stably express firefly luciferase reporter gene under the control of the NF-kB response enhancer element. This cell line is an ideal cellular model for monitoring the activation of NFkB pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human NRF2/ARE Luciferase Reporter Cell Line-HepG2

ABC-RC0035 AcceGen 1 vial Ask for price
Description: Human NRF2/ARE Luciferase Reporter Cell Line-HepG2 is derived from Human Liver cancer, and stably express firefly luciferase reporter gene under the control of NRF2/ARE response element.This cell line is an ideal cellular model for monitoring the activation of Antioxidant response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Hippo Pathway TEAD Luciferase Reporter MCF7 cell line

60618 BPS Bioscience 2 vials 2445 EUR
Description: The TEAD Reporter - MCF7 cell line contains the firefly luciferase gene under the control of TEAD responsive elements stably integrated into the human breast cancer cell line, MCF7. Inside the cells, basal unphosphorylated YAP/TAZ remains in the nucleus and induces the constitutive expression of luciferase reporter. The cell line is validated for the inhibition of the expression of luciferase reporter by the activators of the Hippo pathway.

Human NRF2/ARE Luciferase Reporter Cell Line-HEK293

ABC-RC0034 AcceGen 1 vial Ask for price
Description: Human NRF2/ARE Luciferase Reporter Cell Line-HEK293 is derived from Human embyronic kidney,and stably express firefly luciferase reporter gene under the control of NRF2/ARE response element.This cell line is an ideal cellular model for monitoring the activation of Antioxidant response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human INF-a/ISRE Luciferase Reporter Cell Line- HeLa

ABC-RC0019 AcceGen 1 vial Ask for price
Description: Human INF-a/ISRE Luciferase Reporter Cell Line- HeLa is derived human cervical cancer, and stably express firefly luciferase reporter gene under the control of IFN-α/ISRE response element. This cell line is an ideal cellular model for monitoring the activation of TYK2 and JAK1 response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line

78363 BPS Bioscience 2 vials 11095 EUR
Description: B2M (Beta-2-Microglobulin) has been genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells. Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity.

Human CHOP Luciferase Reporter Cell Line-Mia-Paca2

ABC-RC0007 AcceGen 1 vial Ask for price
Description: Human CHOP Luciferase Reporter Cell Line-Mia-Paca2 is derived from human pancreatic cancer, and stably express firefly luciferase reporter gene under the control of the Unfolded Protein Response, ER stress response element. This cell line is an ideal cellular model for monitoring the activation of Unfolded Protein Response, ER stress Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human ELK-TAD Luciferase Reporter Cell Line-HEK293

ABC-RC0010 AcceGen 1 vial Ask for price
Description: Human ELK-TAD Luciferase Reporter Cell Line-HEK293 is derived human embryonic kidney, and stably express firefly luciferase reporter gene under the control of ELK response element. This cell line is an ideal cellular model for monitoring the activation of MAPK response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Hamster TCF/LEF Luciferase Reporter Cell Line- CHO-K1

ABC-RC0003 AcceGen 1 vial Ask for price
Description: Hamster TCF/LEF Luciferase Reporter Cell Line- CHO-K1 is derived from Chinese Hamster Ovary, and stably express firefly luciferase reporter gene under the control of TCF/LEF response element. This cell line is an ideal cellular model for monitoring the activation of Wnt /b-catenin Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Mouse NRF2/ARE Luciferase Reporter Cell Line-NIH 3T3

ABC-RC0070 AcceGen 1 vial Ask for price
Description: Mouse NRF2/ARE Luciferase Reporter Cell Line-NIH 3T3 is derived from Mouse fibroblast, and stably express firefly luciferase reporter gene under the control of the NRF2/ARE response element. This cell line is an ideal cellular model for monitoring the activation of Antioxidant Response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

AP1 Luciferase Reporter Lentivirus (JNK Signaling Pathway)

79823 BPS Bioscience 500 µl x 2 795 EUR
Description: The stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) family of proteins includes mitogen-activated protein kinases (MAPKs) that are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun. c-Jun then binds to the activator protein-1 (AP1) response element and induces AP1 transcription.
The AP1 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by AP1 response element located upstream of the minimal TATA promoter. After transduction, activation of the JNK signaling pathway and AP1 mediated activity in the target cells can be monitored by measuring the luciferase activity.

Human AR Luciferase Reporter Cell Line-MDA-MB-453

ABC-RC0005 AcceGen 1 vial Ask for price
Description: Human AR Luciferase Reporter Cell Line-MDA-MB-453 is derived from human breast cancer,and stably express firefly luciferase reporter gene under the control of the AR response element. This cell line is an ideal cellular model for monitoring the activation of Androgen Receptor Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human GR Luciferase Reporter Cell Line-MDA-MB-453

ABC-RC0015 AcceGen 1 vial Ask for price
Description: Human GR Luciferase Reporter Cell Line-MDA-MB-453 is derived from human breast cancer, and stably express firefly luciferase reporter gene under the control of the GR response element. This cell line is an ideal cellular model for monitoring the activation of Glucocorticoid Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Human NFkB Luciferase Reporter Cell Line- MDA-MB-231

ABC-RC0026 AcceGen 1 vial Ask for price
Description: Human NFkB Luciferase Reporter Cell Line- MDA-MB-231 is derived from Human breast cancer, and stably express firefly luciferase reporter gene under the control of NFkB response element. This cell line is an ideal cellular model for monitoring the activation of NFkB response Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Minicircle Single Reporter: EF1 Luciferase DNA (30 µg)

BLIV511MC-1 SBI 30 ug 652 EUR

Human SMAD/TGFbeta Luciferase Reporter Cell Line-HepG2

ABC-RC0039 AcceGen 1 vial Ask for price
Description: Human SMAD/TGFbeta Luciferase Reporter Cell Line-HepG2 is derived from human liver cancer, and stably express firefly luciferase reporter gene under the control of the SMAD 2/3 response element. This cell line is an ideal cellular model for monitoring the activation of TGFbeta pathway Receptor Signaling Pathway triggered by stimuli treatment, enforced gene expression and gene knockdown.

Luciferase HeLa Reporter Cell Line-Negative Control

ABC-RC0062 AcceGen 1 vial Ask for price
Description: Luciferase HeLa Reporter Cell Line-Negative Control has been stably transfected with pTA-luciferase reporter vector, which contains no binding sites, a minimal promoter upstream of the firefly luciferase coding region.

Single-Luciferase (Renilla) Reporter Assay Kit

20-abx298011 Abbexa
  • 548.40 EUR
  • 322.80 EUR
  • 200 rxns
  • 50 rxns

Anti-BCMA CAR Jurkat/NFAT (Luciferase) Reporter Cell Line

79694 BPS Bioscience 2 vials 9400 EUR
Description: The anti-BCMA CAR Jurkat/NFAT-luciferase reporter cell line is a stable cell line made from the anti-BCMA scFV CAR lentivirus (BPS Bioscience #79701). It has been validated for anti BCMA-CAR expression by FACS, and for functional activation stimulated by both soluble BCMA protein (BPS Bioscience #79467) and BCMA/CHO target cells (BPS Bioscience #79500).

NFAT Luciferase-eGFP Reporter Lentivirus-78656-G

78656-G BPS Bioscience 500 µl x 2 835 EUR
Description: The NFAT Luciferase-eGFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and eGFP cassette driven by the NFAT response element located upstream of the minimal TATA promoter (Figure 1) and a puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or eGFP expression.

NFAT Luciferase-eGFP Reporter Lentivirus-78656-P

78656-P BPS Bioscience 500 µl x 2 835 EUR
Description: The NFAT Luciferase-eGFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and eGFP cassette driven by the NFAT response element located upstream of the minimal TATA promoter (Figure 1) and a puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or eGFP expression.

Luciferase HepG2 Reporter Cell Line-Negative Control

ABC-RC0063 AcceGen 1 vial Ask for price
Description: Luciferase HepG2 Reporter Cell Line-Negative Control has been stably transfected with pTA-luciferase reporter vector, which contains no binding sites, a minimal promoter upstream of the firefly luciferase coding region.

TCR/B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line

78557 BPS Bioscience 2 vials 16695 EUR
Description: This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells to generate the TCR Knockout NFAT Luciferase Reporter Jurkat cell Line (BPS Bioscience #78556). These TCR knockout cells were used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing. _x000D_Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity. 

These genomes could be observed in retinal cells within 3 hours of AAV subroeting delivery, were about full length and correlated with a vector expression in photoreceptors and retinal pigment epithelium. Saber-fish has easily detected AAV genomes in the liver and muscles according to retro-orbital and intramuscular AAV injections, which respectively demonstrate its utility in different tissues. Using Saber-Fish, we also noted that retinal microglia, a type of cell deemed refractory to AAV transduction, is actually infected by several AAV serotypes, but seem to degrade AAV genomes before nuclear location. Our results show that Saber-Fish can be used to visualize in situ AAV genomes, allowing studies on AAV vector biology and tracking cells transduced after vector administration.

Dodaj komentarz